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用Bacillus subtilis进行代谢工程,用于增强p-酸生产和抗菌应用

Junjie Zhang¹, Guodong Zhang¹, Wenhu Zhu²

¹State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530004, China.

Enzyme and microbial technology

|October 17, 2025

概括

改造了Bacillus subtilis以产生高产的p-酸 (p-CA),实现了80倍的增加. 增强的p-CA表现出改善的抗氧化和抗菌特性,提供了一个可持续的生产平台.

科学领域:

生物技术是生物技术.
微生物工程微生物工程
代谢工程是代谢工程.

背景情况:

p-酸 (p-CA) 是一种在食品和药品中使用的有价值的化合物.
目前的p-CA生产依赖于植物提取或化学合成.
使用 Bacillus subtilis 的p-CA 的微生物合成尚未得到充分的研究.

研究的目的:

为了设计一种高产的p-酸生产菌株的细菌.
为了优化发酵条件,提高p-CA的生产.
评估微生物产生的p-CA的生物活动.

主要方法:

为 p-CA 合成设计了一种复合的 Bacillus subtilis 菌株 (PBK).
通过促进体替代,开发出一种高产品种 (PBnprE).
优化发酵参数和基质,以获得最大的p-CA产量.
评估了p-CA提取物的抗菌活性和抗氧化能力 (DPPH,ABTS).

主要成果:

取得了3.81 mg/L的初始p-CA收益率与菌株PBK.
增加p-CA产量至304.04mg/L,使用优化菌株PBnprE (增加80倍).
优化的提取物显示出对S. aureus,P. aeruginosa和E. coli增强的抗菌活性.
显著提高了DPPH (4.81倍) 和ABTS (3.47倍) 的清理能力.

结论:

成功构建了一个高产量的p-CA生产工程 Bacillus subtilis 菌株.
优化的微生物发酵为p-CA生产提供了可扩展和高效的方法.
增强的p-CA表现出强大的抗氧化和抗菌特性.
这种工程菌株作为生产其他二次代谢物的宝贵平台.

关键词:

这种细菌是 Bacillus subtilis.发酵优化优化发酵的优化活动主办人替代 p-库马里克酸是什么

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