在草果成熟期间,FaERF71C通过抑制FaAPX1D来调节维生素C代谢和抗氧化活性
Zhiliang Pan1, Jiahui Liang2, Linlin Xu2
1College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China; Jiangsu Provincial Key Laboratory for Horticultural Crop Genetics and Improvement/ Zhongshan Biological Breeding Laboratory, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, 210014, China.
Plant physiology and biochemistry : PPB
|October 17, 2025
概括
草果的成熟需要复杂的调节. 研究人员发现了一种新的FaERF71C-FaAPX1D通路,通过抑制甲酸盐过氧化酶活性来控制维生素C (Vc) 水平.
科学领域:
- 植物生物化学 植物生物化学
- 分子生物学分子生物学
- 果实成熟 果实成熟
背景情况:
- 亚酸过氧化酶 (APX) 调节L-亚酸 (维生素C,Vc) 的新陈代谢.
- 在草果成熟期间控制APX活性的机制尚不清楚.
研究的目的:
- 研究APX基因在草果Vc积累中的调控作用.
- 确定FaAPX1D的关键调节者,这是成熟期间的主要APX基因.
主要方法:
- 对FaAPX基因的全基因组识别.
- 基因沉默 (VIGS) 和过度表达试验.
- 转录组联合表达分析,Y1H,双LUC和ChIP测定.
主要成果:
- 确定了24个FaAPX基因;FaAPX1D表达与Vc和H2O2.2相关联.
- 沉默FaAPX1D增加了Vc和H2O2水平.
- FaERF71C直接抑制FaAPX1D,促进了VC的积累.
结论:
- 一个新的FaERF71C-FaAPX1D调节模块在草成熟期间控制Vc水平.
- 这一发现为培育具有增强营养价值的草提供了洞察力.
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