功能性微生物注射剂会影响堆肥修改土壤中DNRA和脱路径的平衡
Xu Zhang1, Kunxue Cui1, Xiangyu Chen1
1College of Life Science, Northeast Agricultural University, Harbin, 150030, China.
Journal of environmental management
|October 17, 2025
概括
在有机堆肥中添加分散酸盐降解 (DNRA) 功能性细菌剂显著增加了的产量. 这通过使DNRA途径在脱化上占主导地位来增强土壤肥.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物地质化学生物地质化学
背景情况:
- 溶解性酸盐降解为 (DNRA) 和脱是循环的关键过程.
- 这些途径竞争酸盐基质,影响的可用性.
- 氨酸代谢,特别是氨酸的丰富性,在这种竞争中发挥着作用.
研究的目的:
- 研究DNRA和脱功能细菌剂对有机堆肥的影响.
- 确定这些物质如何影响酸盐利用和生成.
- 了解微生物社区和通路主导地位的转变.
主要方法:
- 有机堆肥实验涉及三个群体:脱细菌 (E1),DNRA细菌 (E2) 和两者 (E3).
- 对同位素进行标记,以追踪酸盐和的转化.
- 功能性基因丰度 (nrfA, nosZ, nirK) 和微生物群落组成的分析.
主要成果:
- E2注射剂显著增加了酸盐 (NO3−-N) 的利用和 (NH4+-N) 的产生.
- 氨积累在E2中最大化,与E2和E3.3相比增加了13.54%.
- 接种E2导致nrfA基因 (DNRA标记物) 的相对丰度增加了八倍,同时抑制了脱基因 (nosZ, nirK).
- 在E2中,DNRA微生物系的丰富性和多样性增加,其中Thermopetrobacter,Thermomonas和Pseudomonas与nrfA相关.
结论:
- 添加DNRA功能细菌 (E2) 在有机堆肥中显著促进DNRA途径.
- 这种转变增强了的生产,这对土壤肥沃至关重要.
- DNRA细菌改变了微生物平衡,有利于DNRA而不是脱,以改善管理.
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