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无间隙的端粒到端粒的基因组组合大理石 (Pseudopleuronectes yokohamae)

Weiwei Zheng^1,2, Aijun Cui^1,2, Zhen Meng^1,2

¹State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, 266071, China.

Scientific data

|October 17, 2025

概括

研究人员已经测序了第一个没有差距的T2T基因组,用于大理石 (Pseudopleuronectes yokohamae),这是一个商业上重要的鱼类. 这种高质量的基因组为了解其进化和识别与经济特征相关的基因提供了必要的资源.

科学领域:

基因组学就是基因组学.
水产养殖是水产养殖的一种方式.
进化生物学进化生物学

背景情况:

大理石 (Pseudopleuronectes yokohamae) 是东北亚一个商业上有价值的平鱼.
缺乏高质量的参考基因组阻碍了进化和功能基因组学研究.

研究的目的:

报告第一个没有差距的T2T基因组组装对大理石鱼.
为未来关于大理石鱼的研究提供一个宝贵的资源.

主要方法:

结合了短读数,PacBio HiFi长读数,ONT超长读数和Hi-C数据用于基因组组.
将基因组组装在24个染色体上,并确定了具有端粒的基因组.
使用BUSCO和读取映射比率评估基因组完整性.

主要成果:

产生了 582.73 Mb 的无间隙 T2T 基因组组合,具有 26.29 Mb 的 N50 结合.
成功将基因组组装到24个染色体上,识别了48个端粒.
实现了99.29%的BUSCO完整性和>99.89%的读取映射比率,表明了高质量.
确定了121.02 Mb的重复元素和22778个编码蛋白质的基因.

结论:

首个高质量,没有缺口的T2T基因组已经成功生成了.
这种基因组组合为进化基因组学研究提供了基础资源.
它将有助于识别与大理石鱼经济特征相关的关键基因.

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FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...