与赤裸裸的白金线圈相比,在水合线圈上较早和更大的血管内皮细胞增殖:一个体外研究研究
Xiaojie Ding1, Minglei Wang2, Chao Zhang3
1Department of Neurosurgery, Shanghai Putuo People's Hospital, Tongji University, #1291 Jiangning Road, Shanghai, 200060, China.
Scientific reports
|October 17, 2025
概括
在动脉瘤线圈上,HydroCoil栓塞系统 (HES) 促进了比裸线圈更快的内皮细胞生长. 然而,HydroCoils也显示较早的细胞死亡,影响动脉瘤愈合.
科学领域:
- 生物医学工程 生物医学工程
- 血管生物学 血管生物学
- 材料科学 材料科学 材料科学
背景情况:
- 动脉瘤治疗通常涉及栓塞线圈以促进血栓形成和愈合.
- 栓塞性物质的内皮化对于长期修复动脉瘤和减少复发至关重要.
- 液压圈栓塞系统 (HES) 旨在改善动脉瘤封闭和愈合.
研究的目的:
- 为了比较HydroCoils和裸线圈之间的内皮细胞粘附和增殖.
- 评估线圈表面特性对血管内皮细胞行为的影响.
- 评估不同类型的栓塞线圈上的细胞活力和PECAM-1表达.
主要方法:
- 使用了bEnd.3小鼠脑血管内皮细胞,培养在HydroCoils和裸线圈上.
- 在多个时间点 (D0-D21) 量化细胞脱离以生成生长曲线.
- 进行了活细胞/死细胞染色和PECAM-1免疫光检测,以评估细胞活力和内皮细胞标记物表达.
主要成果:
- 在第3天,HydroCoils显示出大量的扩散峰值,在第7天稳定下来.
- 裸体线圈在第14天表现出延迟的增殖峰值,细胞数量较低.
- 活体/死体染色表明细胞粘附于裸线圈,但对水电线圈的粘附性很小.
- 在HydroCoils上,PECAM-1表达明显较弱,而在裸体白金线圈上则明显较弱.
结论:
- 与裸体白金线圈相比,HydroCoils支持更快的内皮细胞增殖和更高的细胞密度.
- 尽管最初的生长速度更快,但HydroCoils似乎会在内皮细胞中诱导早期的亡阶段.
- 这些发现表明,用于动脉瘤治疗的HydroCoils和裸线圈之间存在差异性的生物相容性和愈合潜力.
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