在环境pH值下从湖水中提取的-DOM复合物的超高分辨率质谱分析
Ghulam Hussain Qasim1, Lisa Harris2, Vaughn Mangal2
1Department of Civil and Environmental Engineering, Syracuse University, Syracuse, NY, 13244, USA. ghqasim@syr.edu.
Analytical and bioanalytical chemistry
|October 17, 2025
概括
这项研究开发了一种固相提取方法,以在环境pH下分析溶解有机物 (Hg-DOM) 复合物. 这种新方法可以更好地描述水生生态系统中的Hg-DOM成分.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 水生化学 水生化学
背景情况:
- 用溶解有机物 (Hg-DOM) 复杂的表征对于了解水生系统中的命运至关重要.
- 低度和酸性提取条件限制了当前的Hg-DOM分析.
- 超高分辨率质谱是识别这些复合物的关键.
研究的目的:
- 开发和验证固体相提取 (SPE) 方法,以在环境pH下进行Hg-DOM复合物预缩.
- 为了在提取过程中保持Hg-DOM复合物的完整性,以便进行准确的表征.
- 为了比较PPL和C18 SPE弹对于Hg-DOM提取的有效性.
主要方法:
- 在环境pH下使用PPL和C18弹进行固体相提取 (SPE).
- 在Hg-DOM复合物的预度.
- 使用超高分辨率里埃转换离子循环子子共振质谱法 (FT-ICR-MS) 进行识别和表征.
主要成果:
- 无论是PPL还是C18弹都有效地提取了Hg-DOM复合体,而没有显著的Hg损失.
- C18弹有选择地提取了具有较短碳链的非极性形Hg-DOM复合体 (16-18).
- PPL弹提取了更广泛的Hg-DOM复合物,包括具有较长碳链的异形和芳香化合物 (17-25).
结论:
- 在环境pH下,SPE保留了Hg-DOM复合物的组成,克服了酸性提取的局限性.
- 与优化的SPE相结合的FT-ICR-MS提供了一种强大的方法,用于详细的Hg-DOM分析.
- 这种方法提高了对水生环境中的物种化,生物可用性和转变的理解.
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