相关实验视频

Updated: Jan 14, 2026

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

在Medicago sativa L中对CPP转录因子家族的全基因组识别和表达模式分析

Xinyue Ma^1,2, Xianyang Li^1,2, Li Zhao^1,2

¹College of Life Science and Technology, Harbin Normal University, Harbin, 150025, China.

BMC plant biology

|October 17, 2025

概括

在Medicago sativa L.中识别和分析了富含氨酸的多类似蛋白 (CPP) 基因家族. 这些 MsCPP 基因对植物生长,发育和对干旱和盐度等环境压力的反应至关重要.

科学领域:

植物分子生物学植物分子生物学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

富含氨酸的多类似蛋白 (CPP) 基因家族调节了植物的生殖发育.
以前对CPP基因的表征存在于像Arabidopsis thaliana和Glycine max.这样的物种中.
在Medicago sativa L.中,CPP基因家族仍然没有表征.

研究的目的:

确定和全面分析Medicago sativa L.中的CPP基因家族.
研究 MsCPP 基因在生长,发育和应激反应中的潜在作用.

主要方法:

对Medicago sativa L.基因组的生物信息分析.
对 MsCPP 蛋白质的遗传学分析.
对促进体cis作用元素和基因表达模式 (转录组学,RT-qPCR) 的分析.

主要成果:

在Medicago sativa L.中发现了31个CPP基因家族成员 (MsCPP1-MsCPP31).
遗传学分析揭示了五个子组,它们的图案和结构都保留了.
超过三分之二的MsCPP基因含有干旱诱导性元素,许多基因对干旱,寒冷和盐的压力做出反应.

结论:

MsCPP基因家族在Medicago sativa L.的生长,发育,激素信号和非生物应激适应方面发挥着至关重要的作用.
这项研究提供了对Medicago sativa L.环境适应的基础分子机制的基础见解.

关键词:

梅迪卡戈·萨蒂瓦 (Medicago sativa) L. L. 是一种植物.MsCPP基因家族的基因家族表达模式分析表达模式分析进行全基因组分析.

更多相关视频

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

Lateral Root Inducible System in Arabidopsis and Maize

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

相关概念视频

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

Combinatorial Gene Control

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...

Constitutive and Regulated Gene Expression

Constitutive and Regulated Gene Expression

Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...