循环时钟蛋白BMAL1和CLOCK调节HSV-1通过NECTIN-1进入神经细胞
JinJie Tang1, Meifang Pan2, Jiahui Liu1
1State Key Laboratory of Traditional Chinese Medicine Syndrome, Chinese Medicine Guangdong Laboratory (Hengqin), Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Guangzhou, China.
Virology journal
|October 17, 2025
概括
生物钟通过控制NECTIN-1的表达来调节简单疹病毒1型 (HSV-1) 的进入. 核心时钟因子BMAL1和CLOCK针对NECTIN-1,为针对HSV-1的昼夜抗病毒疗法提供了潜在的潜力.
科学领域:
- 神经病毒学 神经病毒学
- 时间生物学 时间生物学
- 分子生物学分子生物学
背景情况:
- 简单疹病毒1型 (HSV-1) 建立了终身潜伏期,并可能导致致命的疹脑炎.
- 了解HSV-1调控对于开发有效治疗方法至关重要.
研究的目的:
- 确定调节HSV-1感染的关键昼夜钟基因.
- 阐明这种调节背后的分子机制.
主要方法:
- 对小鼠脑干和海马体的转录组分析.
- 血清冲击细胞模型用于评估NECTIN-1表达和HSV-1感染.
- 露西法酶记者测定和染色体免疫沉降 (ChIP) 为了研究转录调节.
主要成果:
- 作为HSV-1入口受体的NECTIN-1在小鼠大脑和海马体中表现出节奏表达.
- HSV-1的DNA拷贝数与NECTIN-1的表达水平相关.
- BMAL1 和 CLOCK 通过与其促进体结合来提高NECTIN-1 的调节; 抑制 CLOCK 可减少 HSV-1 感染.
结论:
- 昼夜时钟节奏地控制HSV-1通过NECTIN-1进入.
- BMAL1和CLOCK是针对HSV-1的昼夜抗病毒策略的潜在治疗点.
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