在癌细胞和种子表现上的Auxin衍生碳点的生物成像应用和增长促进行为
Ghasem Noorkhajavi1,2, Meysam Najaflou3, Hana Farsinezhad2
1Cancer Immunology and Immunotherapy Research Center, Ardabil University of Medical Sciences, Ardabil, Iran.
Journal of biological engineering
|October 18, 2025
概括
环保的辅酶衍生碳点 (Aux-CDs) 在医学和农业中具有双重用途. 这些Aux-CD对于生物成像癌细胞和增强小麦种子发芽是有效的,为各种应用提供了一个有前途的纳米材料.
科学领域:
- 纳米材料科学 科学 纳米材料科学
- 生物医学工程 生物医学工程
- 农业科学 农业科学
背景情况:
- 为生物医学和农业应用开发具有双重功能的新型纳米材料至关重要.
- 碳点 (CD) 具有独特的光发光特性和生物相容性,使其对各种用途具有吸引力.
- 植物激素auxin可以作为合成功能化碳点的前体.
研究的目的:
- 通过环保的一步热水方法合成由auxin衍生的碳点 (Aux-CDs).
- 评估Aux-CDs在癌细胞生物成像和农业种子增强中的双重功能.
- 描述合成的Aux-CDs的物理化学特性和生物相容性.
主要方法:
- 从auxin和水中单步液热合成Aux-CDs.
- 使用UV-Vis,泽塔电位,光发光,SEM,FT-IR和TEM进行物理化学表征.
- 通过MTT测定和光成像,评估Aux-CDs在4T1癌细胞中的细胞毒性和内化.
- 评估Aux-CDs对小麦种子 (Drum和Ehsan品种) 发芽和生长的作用,通过纳米化和光显微镜.
主要成果:
- 辅助CD成功合成了43%的量子产量和大约10纳米的大小.
- 在4T1癌细胞中Aux-CDs的低细胞毒性,观察到内部化和生物成像的潜力.
- 在低度 (1μg/mL) 的小麦种子发芽率和性能指数显著改善.
- 在小麦种子的生长部分观察到光,证实了Aux-CD的吸收和运输.
结论:
- 一步式热水合成为生产Aux-CD提供了一种环保和经济高效的生产途径.
- 辅助CD显示出出色的生物相容性和适合生物成像应用的多功能特性.
- 辅助CD显示出在农业环境中增强植物生长和性能的巨大潜力.
- 辅助CD代表了一个有前途的多功能纳米材料,用于综合生物医学和农业研究.
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