在肝脏和脏中对纳米粒子吸收的先天性免疫反应模仿了病原体感染
Bertha A Brodin1, Giovanni M Saladino1, Hans M Hertz1
1Department of Applied Physics, KTH Royal Institute of Technology, Stockholm, Sweden.
Nanomedicine (London, England)
|October 18, 2025
概括
系统的纳米颗粒 (MoNPs) 的管理导致肝脏和脏的炎症. 这些纳米粒子的涂层有效地降低了它们的毒性,并调节了免疫反应.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术纳米技术
- 免疫学 免疫学 免疫学
背景情况:
- 系统管理的纳米粒子 (NP) 通常保留在肝脏和脏中.
- 这些器官中的组织巨细胞的快速细胞化会导致炎症.
- 了解NP-免疫细胞相互作用对于生物医学应用至关重要.
研究的目的:
- 为了研究纳米颗粒 (MoNPs) 的体内分布.
- 检查NP-巨细胞相互作用和肝脏,脏和肺部的生理反应.
- 评估二氧化涂层对NP毒性和免疫反应的影响.
主要方法:
- 在临床前的模型中,对MoNPs进行静脉注射.
- 在体内纳米粒子跟踪的X射线光 (XRF) 成像.
- 分析免疫细胞群 (库夫弗细胞,NK细胞),氧化应激标志物,基因表达和巨细胞表型.
- 在NP吸收中调查脏巨细胞的参与 (CD169/Siglec1).
主要成果:
- 在静脉注射后,MoNPs在肺部,肝脏和脏中暂时积累.
- 吸收NP触发了库普弗细胞,NK细胞,氧化应激和炎症的增加.
- 巨细胞表型转向抗炎状态,表达免疫调节基因.
- CD169/Siglec1脏巨细胞参与了NP的吸收,这表明了二次免疫反应.
- 对MoNPs的涂层降低了观察到的毒性.
结论:
- 肝脏和脏对NP吸收的先天免疫反应类似于对微生物感染的反应.
- 对NP的二次免疫反应可以通过CD169/Siglec1大细胞在脏中启动.
- 金属NP的涂层可以减弱免疫反应,降低毒性.
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