埃舍里希亚大肠杆菌长期存活在西兰醇 - 酸盐凝中的形式和机制
O A Galuza1,2, G I El'-Registan3, A V Khramova3
1Federal Research Center for Biotechnology, Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, 119071, Russia. olesya_galuza@mail.ru.
Archives of microbiology
|October 18, 2025
概括
锡拉诺酸盐凝 (SHG) 增强了细菌的长期存活. 在SHG中使大肠杆菌不活动,促进了低代谢状态和休眠形式,显著增加了生物技术应用的细胞活力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物催化剂是一种生物催化剂.
背景情况:
- 之前,西醇酸盐凝 (SHG) 已经证明了有氧碳化合物氧化剂和乳酸细菌的长期存活率.
- 大肠杆菌 (E. coli) K-12 (MG1655) 呈现出呼吸和发酵代谢,使其成为研究固定效应的合适模型.
- 长期的微生物稳定对于各种生物技术过程至关重要.
研究的目的:
- 调查SHG固定和随后存储对大肠杆菌生存的影响.
- 了解大肠杆菌在SHG固定后的适应性反应.
- 为了确定SHG制剂中使用的有机酸对细胞活性的影响.
主要方法:
- 用不同有机酸制备的西兰醇 - 酸盐凝 (SHG) 固定大肠杆菌K-12 (MG1655).
- 在室内条件下长期储存固定和非固定 (对照) 大肠杆菌培养物,长达5个月.
- 监测细胞活力,代谢状态 (二氧化碳排放,酸消耗) 和幸存人群的特征 (低代谢,休眠,持续细胞,抗生素耐受性).
主要成果:
- SHG固定诱导了大肠杆菌的适应性反应,其特征是低代谢状态,增长缓慢,并过渡到休眠形式.
- 生存效率受到凝过程中使用的有机酸类型的显著影响,,,酸或乳酸产生了最高的可活细胞标位 (比对照细胞高50-100倍).
- 在SHG中长期储存的大肠杆菌表现出低代谢状态 (代谢率低四个数量级),耐热性低,持续性细胞丰富,抗生素耐受性增加.
结论:
- SHG固定有效地稳定大肠杆菌,通过诱导的低代谢和休眠促进生存.
- 在SHG制备过程中选择有机酸对于优化细菌生存能力至关重要.
- 观察到的SHG不动化的大肠杆菌的特征表明其在生物技术中的应用潜力很大,特别是微生物生物催化剂的长期稳定.
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