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Updated: Jun 27, 2026

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
10:01

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Published on: July 30, 2020

使用HILO显微镜成像和基因工程来量化活细胞中相分离的冷凝物质的协议

Axel Espinoza1, Steven Ingersoll2, Xiaojun Ren3

  • 1Department of Integrative Biology, University of Colorado Denver, Denver, CO 80217-3364, USA.

STAR protocols
|October 18, 2025
PubMed
概括

这项研究引入了一种新的方法来测量表观遗传因素如何通过活细胞的相位分离聚集. 该协议使用先进的显微镜和遗传工具来可视化和量化基因组组织的这一关键过程.

关键词:
生物物理学的生物物理.克里斯普尔是什么意思?克里斯普尔是什么意思?细胞生物学 细胞生物学显微镜 显微镜是指使用显微镜.

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Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.