综合稳定同位素追踪和酸性氧化物减少 (SIT-APIR) 方法用于在不同环境中确定微生物化率
Jiapeng Wu1, Yuexi Ma1, Xiaoyi Li1
1Institute of Environmental Research at Greater Bay Area, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, P. R. China.
Analytical chemistry
|October 18, 2025
概括
一种新的稳定同位素追踪和酸性氧化物减少 (SIT-APIR) 方法准确地测量了氨和酸盐的氧化率. 这种技术增强了对各种生态系统中化过程的理解.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 分析化学 分析化学
背景情况:
- 准确量化氨氧化率 (Ra) 和酸盐氧化率 (Rn) 对于理解化至关重要.
- 有限的方法可以同时测量Ra和Rn.
研究的目的:
- 开发和验证一种用于同时确定Ra和Rn的新方法.
- 提高化率测量的准确性和效率.
主要方法:
- 使用15NH4+和15NO2-标记剂进行稳定同位素追踪.
- 酸性化降解 (APIR) 用于将15NO2和15NO3转化为15NO.
- 使用膜入口质谱法 (MIMS) 测量15NO.
主要成果:
- 该SIT-APIR方法显示了高精度 (例如,0.65%的15NO3-误差) 和稳定性 (例如,5.56%的RSD15NO2-).
- 该方法具有较低的检测极限 (0.1μmol L-1) 和出色的线性 (R2 ≥ 0.994).
- 通过Gompertz模型确定了最佳化时间,该方法适用于水,沉积物和土壤样本.
结论:
- 该SIT-APIR方法为测量化率提供了显著的进步.
- 这种技术为各种环境样本提供了更高的准确性,效率和成本效益.
- 该方法有助于更好地了解各种生态系统中的化过程.
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