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在MITF中识别InDels与子的嘴颜色相关

Tian Hua¹, Rui Pan², Yong Jiang¹

¹College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

Poultry science

|October 18, 2025

概括

遗传分析发现MITF是影响子尾颜色的关键基因. 在MITF中,一个特定的插入 (InDel-4)

科学领域:

动物遗传学动物遗传学
分子生物学分子生物学
量化遗传学量化遗传学

背景情况:

嘴巴颜色是子品种中与色素相关的重要特征.
之前关于子的颜色遗传学的研究集中在单基基突变上,这些突变不足以解释表型变异.
识别结构突变对于理解和选择的颜色特征至关重要.

研究的目的:

确定关键基因和与子嘴颜色形成相关的差异性插入-删除突变 (InDels).
在Runzhou白顶的F2种群上进行全基因组关联研究 (GWAS).
利用基因组选择特征 (FST和 θπ) 来识别候选变异.

主要方法:

在308名F2个体上进行了全基因组关联研究 (GWAS).
基因组选择特征使用FST和 θπ值进行了分析,比较了黑和黄的线.
在部组织中进行了基因型和相对MITF基因表达分析.

主要成果:

通过GWAS确定了两个内在的InDels作为候选变体.
基于基因组选择签名,MITF基因被选择为一个关键候选者.
确定了MITF内突中的1bp插入 (InDel-4) 作为候选位点,黄色嘴是同卵性突变的,黑嘴是同卵性参考.
相对MITF表达在黑嘴 (H2) 与黄嘴 (CV) 相比显著更高.

结论:

MITF在子的黑色素生成和黑色素沉积中发挥着关键作用,从而影响子颜色.
鉴定到的InDel-4变种与黄色和黑色之间的嘴颜色差异显著相关.
InDel-4可以作为一种有价值的分子标记物,用于识别子的颜色.

关键词:

一个子.在InDel,我们可以看到.美国MITF MITF 嘴巴的颜色嘴巴的颜色基因组选择签名基因组选择签名

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