不同的低温保存条件在储存期间对野猪精子质量的影响:精子丰富的部分和整个射精的评估
Paulina Grudzinska1, Chiara Luongo2, Armando Quintero-Moreno1
1Departamento de Fisiología, Facultad de Veterinaria, Campus de Excelencia Mare Nostrum, Universidad de Murcia, Murcia, Spain.
Research in veterinary science
|October 18, 2025
概括
猪精液的低温储存,特别是5°C,有效地保持了用于人工授精 (AI) 的精子质量. 不同的温度协议维持了精子功能,并支持体外受精 (IVF) 的结果,无论射精分数如何.
科学领域:
- 兽医生殖和人工授精 兽医生殖和人工授精
- 精子生物学和冷保存
背景情况:
- 低温猪精液储存是一种抗生素的替代品,可以减少人工授精 (AI) 中的抗菌素耐药性.
- 使用半自动采集系统对整个射精 (WE) 和精子丰富分数 (SRF) 的最佳处理协议需要定义.
研究的目的:
- 评估不同温度储存协议对野猪精子功能的影响.
- 评估精子质量,热应激反应,新陈代谢和体外受精 (IVF) 结果.
主要方法:
- 野猪精液 (SRF和WE) 在15°C,5°C或经受温度变化 (5°C/15°C和15°C/5°C) 保存4天.
- 评估了精子运动性,动力学,膜完整性,线粒体活动,热应激反应,新陈代谢 (OCR,ECAR) 和试管婴儿.
主要成果:
- 精子质量受到5°C的储存和温度变化的轻微影响,但仍保持在最佳范围内.
- 热应激反应和精子代谢在不同组之间没有显著差异.
- 所有的储存协议都支持试管婴儿,而15°C/5°C组的透率更高.
结论:
- 存储在5°C,单独或与15°C相结合,有效地保护了AI的野猪精子功能.
- 这些协议对于人工智能应用来说是实用的,无论收集的射精分数如何.
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