使用微流体纸片和色度像素面积分析方法同时量化饮料中的果糖和糖糖
Chan-Chiung Liu1, To-Lin Chen2, Hsing-Meng Wang2
1Department of Food Science, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
Food chemistry
|October 18, 2025
概括
本研究引入了一种基于纸张的微流体分析装置 (μPAD),用于饮料中的糖的快速定量. 该设备准确测量果糖和糖度,有助于食品质量监测和健康评估.
科学领域:
- 分析化学 分析化学
- 生物医学工程 生物医学工程
- 食品科学 食品科学 食品科学
背景情况:
- 过度摄入果糖和糖糖等糖类与严重的健康问题有关,如肥胖,2型糖尿病和心血管疾病.
- 准确量化饮料中的糖含量对于公共卫生和食品行业的质量控制至关重要.
研究的目的:
- 开发一种快速,高效和具有成本效益的方法,同时量化饮料中的果糖和糖度.
- 使用微流体基于纸张的分析装置 (μPAD) 与图像处理集成用于糖分析.
主要方法:
- 一个μPAD被设计为-乙-酸试剂 (PABR) 和-乙试剂 (PAR) 的分区,分别检测果糖和糖.
- 饮料样品在与试剂和硫酸反应后在37°C进行了6分钟的分析,形成红色复合物.
- 像素面积图像处理分析了复合体的颜色强度,通过校准曲线确定糖度.
主要成果:
- PAD在20-80毫米范围内的同时检测果糖和糖的线性反应.
- 估计的检测极限为果糖的2.8mM和砂糖的4.2mM (R2 = 0.9909).
- 对HPLC的验证显示,商业饮料的检测误差低于6.0%,恢复率高于94.7%.
结论:
- 开发的μPAD提供了一个有希望的,准确和高效的工具,用于饮料中的糖量化.
- 该方法支持食品质量监测和饮料行业的应用,有助于改善健康结果.
- PAD技术与图像处理的整合为快速分析测试提供了一种新的方法.
相关概念视频
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01:03Precipitation Gravimetry
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