生物转化为ursodeoxycholic 酸使用β-cyclodextrin 包括-chenodeoxycholic 酸通过Xanthomonas maltophilia的固定细胞
Zengyan Yang1, Caixia Liu1, Yan Jin1
1School of Life Sciences and Medical Engineering, Anhui University, Key Laboratory of Eco-engineering and Biotechnology of Anhui Province, Hefei, Anhui 230601, China.
Journal of biotechnology
|October 18, 2025
概括
这项研究引入了一种新的持续有氧生物转化系统,用于使用固定Xanthomonas maltophilia细胞合成ursodeoxycholic酸. 这种方法克服了溶解性差和制备挑战,提供了有效的生物转化策略.
科学领域:
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
- 微生物学 微生物学
背景情况:
- 乌尔索多西胆酸对于肝胆道疾病至关重要,但在水溶性和生物制备方面面临挑战.
- 目前存在的ursodeoxycholic酸合成方法受到溶解性差和复杂的制备过程的阻碍.
研究的目的:
- 开发一种持续的有氧生物转化系统,以高效地合成ursodeoxycholic酸.
- 为了解决水溶性差和ursodeoxycholic酸艰难生物制备的局限性.
主要方法:
- 在微球内的Xanthomonas maltophilia细胞的固定.
- 采用氧化/β-环氧化含有复合物来增强基质的溶解性.
- 在连续系统中,优化反应参数,包括流速,流体方向和填充体积.
主要成果:
- 与纯药物相比,开发的包容复合物提高了溶解度122倍.
- 动不动的X. maltophilia细胞表现出稳定的外核心结构和良好的耐磨性.
- 优化的连续反应条件 (1.0mL/分钟流速,反向流体方向,1:1填充量) 允许连续三批次没有转换损失.
结论:
- 成功建立了一种新的持续有氧生物转化系统,用于制备ursodeoxycholic酸.
- 该系统有效地利用动不动的X. maltophilia和通过包含复合体提高基质溶解度.
- 这种生物转化策略为高效生产ursodeoxycholic酸提供了有价值的替代方案.
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