植物对满月光的反应需要SUVH4 HMTase,它可以通过与拼接因子SAP18的相互作用来准活性基因
Jeevan R Singiri1, Naveen-Kumar Yarra1, Sasank Sannidhi1
1French Associates Institute for Agriculture and Biotechnology of Drylands, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben Gurion 84990, Israel.
植物对月光做出反应,影响生长和细胞活动. 表观遗传修饰剂DDM1和SUVH4在调解这些反应中起着关键作用,影响基因表达和蛋白质概况.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 月光对植物的影响往往被忽视.
- 最近的研究表明,月光会影响植物细胞过程和生长.
- 表观遗传调节对于植物发育和环境反应至关重要.
研究的目的:
- 研究阿拉比多普西斯表观遗传修饰剂DDM1和SUVH4在植物对满月光 (FML) 的反应中的作用.
- 阐明植物中月光感知和信号传递的基础分子机制.
主要方法:
- 用于用于DDM1和SUVH4.4的阿拉比多普西斯突变物.
- 分析了植物对FML的反应,包括角开放和节展开.
- 进行蛋白质组分析 (GST pull-down,GFP-TRAP) 来识别蛋白质相互作用.
主要成果:
- WT和ddm1幼苗对FML表现出反应,而suvh4突变体在 skotomorphogenesis 中受损.
- FML暴露改变了WT和ddm1中的基因组组织,蛋白质和代谢物概况,但不是suvh4.4.
- 鉴定了SAP18,一种亡和拼接相关的蛋白质,作为SUVH4相互作用蛋白质,将基因组修饰与拼接因子联系起来.
结论:
- 通过SUVH4 HMTase进行的质子修饰与ASAP复合的剪接因子有关.
- 这种相互作用可能会调解植物的基因沉默和月光信号解释.
- 突出了月光信号传输的新途径,涉及表观遗传和拼接机械.
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