相关实验视频

Updated: Jan 14, 2026

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS

Published on: December 21, 2011

巨细胞衍生的外体体miR-146a-5p调节PNKP/DDOST/JAGN1复合体,以调节动脉样硬化中NETs的形成

Zhen Liu¹, Wei Zhang¹, Yihang Li¹

¹Department of Vascular Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, China.

Cellular signalling

|October 18, 2025

概括

携带miR-146a-5p的巨细胞外体细胞通过PNKP/DDOST/JAGN1通路调节中性粒细胞外细胞陷 (NET) 的形成,为动脉样硬化 (AS) 提供新的治疗点. 这一发现揭示了AS进展中的巨细胞-中性粒细胞相互作用.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
心血管研究研究心血管研究

背景情况:

动脉样硬化 (AS) 的进展与中性粒细胞外细胞陷 (NET) 的形成有关.
在AS中,巨细胞和中性粒细胞之间的精确调节相互作用仍然不完全理解.

研究的目的:

阐明巨细胞衍生的外体在调节动脉样硬化期间的NET形成中的作用.
研究涉及miR-146a-5p及其下游目标的特定分子机制.

主要方法:

在ApoE-/-小鼠中建立了动脉样硬化模型.
从氧化低密度脂蛋白 (ox-LDL) 刺激的巨细胞中分离出来的外体.
使用qRT-PCR,西斑,共免疫沉,RIP和双化酶记者测试对PNKP/DDOST/JAGN1复合物的miR-146a-5p调节进行分析.

主要成果:

巨细胞衍生的外体显示了miR-146a-5p的增加,抑制PNKP和改变DDOST酸化.
这个级联激活了JAGN1依赖的NET形成.
在AS小鼠中,抑制miR-146a-5p降低了NETs,活性氧物种 (ROS),斑块负担和炎症.

结论:

巨细胞外体miR-146a-5p通过PNKP/DDOST/JAGN1轴调节NET的形成.
这一途径在AS病变发生过程中呈现出一种新的机制.
确定了AS治疗的潜在治疗点.

关键词:

在 AS AS AS AS AS 里面.外基因组是外基因组的组成部分.网络网络网络网络在PNKP/DDOST/JAGN1中.在 miR-146a-5p 中使用.

更多相关视频

Synthesis of Monocyte-targeting Peptide Amphiphile Micelles for Imaging of Atherosclerosis

Synthesis of Monocyte-targeting Peptide Amphiphile Micelles for Imaging of Atherosclerosis

Published on: November 17, 2017

相关概念视频

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...