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一种基于同位素稀释-液体色谱-并联质谱的候选参考测量程序,用于定量人尿中的皮质醇
Myriam Ott1, Friederike Bauland2, Daniel Köppl3
1Roche Diagnostics GmbH, Nonnenwald 2, 82377 Penzberg, Germany.
Clinical chemistry and laboratory medicine
|October 19, 2025
概括
一种新的液体染色学-双重质谱法准确地测量尿皮质醇. 这种强大的方法确保可靠的诊断和监测皮质醇相关的条件.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 精确的尿皮质醇测量对于诊断和监测与皮质醇过量或不足相关的条件至关重要.
- 现有的方法可能缺乏可靠的临床应用所需的特异性和准确性.
研究的目的:
- 开发和验证一种选择性和特定的同位素稀释二维心脏切割液体色谱-并联质谱法 (LC-MS/MS) 方法,用于精确的尿皮质醇测定.
主要方法:
- 使用特定的列和移动阶段,采用二维心脏切割LC-MS/MS方法.
- 一个支持的液体提取 (SLE) 协议被用于样本的准备.
- 方法验证包括对准确性,精度,线性,矩阵效应和测量不确定性的评估.
主要成果:
- 该方法实现了皮质醇与干扰化合物的基线分离.
- 证明了出色的线性 (r≥0.999) 和低的变化系数 (CVs在1.6-3.2%) 之间.
- 测量不确定性 (k=2) 为≤6.8%,随着测量增加,显著降至1.7-2.5%.
结论:
- 开发的LC-MS/MS方法是强大的,准确的,可靠的量化尿皮质醇.
- 这种参考测量程序 (RMP) 适合在验证工作范围 (2.00-220 ng/mL) 内的临床使用.
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