ресвератрол 影响细胞活动,诱导细胞亡,并调节 AMPK 信号通路在多发性间皮瘤细胞
Maria Rosa Iaquinta1, Raffaella De Pace2, Assia Benkhalqui1,3
1Department of Medical Sciences, Laboratories of Cell Biology and Molecular Genetics, University of Ferrara, Ferrara, Italy.
复星 (RSV) 显示出作为治疗辅助剂的承诺恶性多层层层瘤 (PM). 这种天然化合物有效地抑制PM细胞活力,迁移和增殖,为癌症治疗提供了新的途径.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 恶性多叶层层层瘤 (PM) 是一种具有有限治疗选择的侵袭性癌症.
- 复星 (RSV),一种天然的多,已证明具有抗癌性质.
- 作为PM的辅助疗法,RSV的潜力需要进一步研究.
研究的目的:
- 评估Resveratrol (RSV) 作为瘤抑制剂和辅助剂在恶性多间皮质瘤 (PM) 细胞系中的疗效.
- 研究RSV对PM细胞影响的分子机制.
- 评估RSV对细胞活力,细胞亡,迁移和信号通路的影响.
主要方法:
- 用RSV对PM细胞系 (MSTO-211H和IST-MES2) 的治疗.
- 使用MTT试验和活/死染色的细胞活性的评估.
- 通过Annexin V-PI和Caspase-3/7染色对亡的分析.
- 对细胞迁移,E-cadherin表达和β-catenin局部化的评估.
- 测量细胞内水平.
- 关键细胞通路的基因表达分析,包括Wnt/β-catenin和AMPK信号传递.
主要成果:
- 在PM细胞系,特别是MSTO-211H中,RSV显著降低了活力,并诱导了亡.
- RSV抑制了两个PM细胞系的迁移能力.
- RSV治疗影响了细胞形态,减少了E-cadherin表达,并减少了核β-catenin,表明Wnt/β-catenin信号的减弱.
- 与细胞粘附,增殖,代谢活性和AMPK信号传递相关的RSV调节的基因表达.
- 在IST-MES 2细胞中,RSV降低了线粒体和细胞质水平.
结论:
- Ресвератрол 显示出对恶性质间皮瘤细胞具有显著的抗瘤作用.
- RSV作为一种潜在的辅助剂,抑制增殖,诱导亡,减少迁移.
- RSV的机制涉及关键信号通路的调节,包括Wnt/β-catenin和AMPK,使其成为PM治疗的有希望的候选人.
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