Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jan 14, 2026

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
10:00

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Published on: January 12, 2015

15.1K

在斑马鱼心脏发育过程中阐明心肌细胞状态转换,使用转录组动力学.

Dongxu Jin1, Yihao Zhu1, Yao Zu2

  • 1International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.

Biochemical and biophysical research communications
|October 19, 2025
PubMed
概括

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Magnetocardiography in the diagnosis of suspected ANOCA: a case series.

Frontiers in cardiovascular medicine·2026
Same author

Targeting non-canonical antigens unlocks functional T-cell responses in renal cell carcinoma.

Journal for immunotherapy of cancer·2026
Same author

Closed-Loop Radiative Cooling Mulch Upcycled From Agricultural Residues for Efficient Soil Heat-Water Stress Mitigation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Opioid-free Anesthesia for Enhancing Recovery Quality at Postoperative 24 Hours: A Systematic Review and Meta-analysis.

Pain physician·2026
Same author

DEHA-induced hepatotoxicity identified from screening food packaging non-phthalate plasticizers: An integrative computational, multi-omics, and experimental exposure study.

Chemico-biological interactions·2026
Same author

Neuromuscular control mechanism during posture-cognition dual task in mild cognitive impairment: a concurrent fNIRS-sEMG-COP study.

GeroScience·2026

这项研究使用斑马鱼在心脏发育过程中绘制基因表达图,识别了与先天性心脏病 (CHD) 相关的 smtnl1 等关键基因. 了解这些遗传驱动因素对于心血管疾病研究至关重要.

科学领域:

  • 发展生物学 发展生物学
  • 遗传学 遗传学 是一个
  • 心血管研究研究心血管研究

背景情况:

  • 先天性心脏病 (CHD) 是最常见的出生缺陷,但其遗传基础尚未完全理解.
  • 研究心脏发育的分子机制对于确定心脏病的原因至关重要.

研究的目的:

  • 用斑马鱼模型研究心脏形态发生过程中的转录动力学.
  • 为了确定关键的基因和分子途径,涉及到心脏发育和心脏病发病的病原体.

主要方法:

  • 用斑马鱼胚胎进行心脏形态生成研究.
  • 在多个胚胎阶段 (24-48 hpf) 进行了批量RNA测序 (Bulk RNA-seq) 和单细胞RNA测序 (scRNA-seq).
  • 采用加权基因共同表达网络分析和伪时间分析.

主要成果:

  • 与特定的心脏形态遗传事件 (心脏管形成,腔室形成,循环,门形成) 相关的基因表达动态.
  • 确定了一个关键的基因模块 (M5),与心肌细胞在发育过程中的状态过渡有关.
  • 发现了像1 (smtnl1) 这样的滑素作为一个枢纽基因;它的淘汰导致斑马鱼的心脏功能障碍.

结论:

关键词:
心脏的发展心脏的发育单细胞转录组学 单细胞转录组学时间序列时间序列文字转录学 (Transcriptomics) 是一个学科.斑马鱼是一种斑马鱼.

更多相关视频

Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function
07:07

Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function

Published on: January 5, 2024

1.8K
Isolation and Characterization of Single Cells from Zebrafish Embryos
09:25

Isolation and Characterization of Single Cells from Zebrafish Embryos

Published on: March 12, 2016

15.1K

相关实验视频

Last Updated: Jan 14, 2026

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
10:00

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Published on: January 12, 2015

15.1K
Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function
07:07

Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function

Published on: January 5, 2024

1.8K
Isolation and Characterization of Single Cells from Zebrafish Embryos
09:25

Isolation and Characterization of Single Cells from Zebrafish Embryos

Published on: March 12, 2016

15.1K
  • 概述了胚胎心脏发育的特定阶段的分子驱动因素.
  • 确定了包括smtnl1在内的候选基因,用于进一步研究心血管疾病的病原性.
  • 突出了斑马鱼模型在研究复杂的先天异常方面的实用性.