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超声波化调节抗菌声动疗中的细胞内ROS和基因表达

Xinyu Li¹, Fengmeng Teng², Fengmin Wu¹

¹Department of Applied Physics, School of Science, Harbin University of Science and Technology, Harbin 150080, China.

Ultrasonics sonochemistry

|October 19, 2025

概括

抗菌声动疗法 (aSDT) 使用超声来杀死细菌. 黄素增强的aSDT主要通过机械细胞破坏和由活性氧物种引发的亡来起作用,提供了一种新的抗菌方法.

科学领域:

生物医学工程生物医学工程
抗微生物疗法抗微生物疗法
纳米技术纳米技术

背景情况:

抗菌声动疗法 (aSDT) 是一种使用超声激活进行抗菌治疗的非侵入性方法.
黄素 (CUR) 被探索为一种声敏剂,以提高SDT的疗效.
了解SDT的机制对于优化其应用至关重要.

研究的目的:

研究黄素增强抗菌声动疗 (aSDT) 的机制.
评估反应性氧物种 (ROS),微气泡化和SDT中的亡的作用.
在遗传和形态层面分析aSDT对Pseudomonas aeruginosa的影响.

主要方法:

细菌活力测定和生物质量定量.
流细胞计用于亡检测和细胞内ROS测量.
RNA测序 (RNA-seq),qRT-PCR和扫描电子显微镜 (SEM) 用于基因表达和形态分析.

主要成果:

aSDT疗效与化强度之间存在剂量依赖的关系.
主要机制包括超声波诱导的机械膜破坏和通过细胞外ROS的亡.
aSDT调节了P. aeruginosa的基因表达,破坏了生物膜,并损害了氧化还原平衡.

结论:

黄素增强的aSDT通过机械破坏和ROS诱导的亡有效地向细菌.
超声波化和声敏剂的协同效应是SDT杀菌作用的关键.
aSDT提供了一种有希望的策略,通过破坏细胞完整性和平衡来对抗细菌感染.

关键词:

抗菌声动力学疗法抗菌声动力学疗法洞穴形成洞穴形成基因表达基因表达基因表达细胞内ROS是细胞内ROS.

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