益生菌的分层武装以提高生存能力,以协同缓解性结肠炎
Zuwei Liu1, Lei Feng1, Qin Huang1
1State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, 330047, China.
层次化装甲的益生菌,Lactobacillus reuteri (Lr) 涂有β-葡萄糖 (GN) 并封装在大豆蛋白分离物 (SPI) 中,显示出对性结肠炎 (UC) 治疗的增强活力和有效性.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 胃肠病学 胃肠病学
背景情况:
- 口服益生菌对性结肠炎 (UC) 有潜在的益处,但胃肠道疾病和不稳定性限制了它们的有效性.
- 现有的保护策略,如单细胞涂层和微封装,需要进一步改进,以获得最大的治疗效果.
研究的目的:
- 开发一种对Lactobacillus reuteri (Lr) 的等级化装甲策略,使用β-葡萄糖 (GN) 涂层和大豆蛋白分离物 (SPI) 微封装.
- 为了提高益生菌活力和治疗疗效的性结肠炎 (UC) 管理.
主要方法:
- 集成的单细胞涂层Lr与GN和基于SPI的微封装,以创建Lr@GN@SPI.
- 评估了Lr@GN@SPI对胃肠道过境挑战的保护效果.
- 评估了Lr的受控释放以及肠道中短链脂肪酸 (SCFA) 的协同效应.
- 在UC小鼠模型中研究了Lr@GN@SPI的治疗和预防作用.
主要成果:
- 与单层保护相比,等级装甲 (Lr@GN@SPI) 显著提高了Lr的生存能力.
- 在肠道中,SPI降解和GN代谢促进了受控的Lr释放和SCFA生产.
- 在小鼠模型中,口服Lr@GN@SPI有效地缓解了UC,通过恢复粘膜完整性,调节炎症和重新平衡肠道微生物群.
结论:
- 层次化装甲策略为益生菌提供了对恶劣胃肠道疾病的优越保护.
- 这种方法使益生菌和SCFA之间能够产生协同治疗效果,以加强UC治疗.
- 使用食品级基质,这种方法为管理肠道健康障碍和改善益生菌输送提供了一个有希望的途径.
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