连续流量优于序列批量操作:对在低氨,寡性条件下丰富完整的氨氧化细菌的机械洞察
Wenni Xi1, Lei Cheng1, Xiaoyu Fang1
1School of Resources and Environmental Engineering, Anhui University, Hefei 230601, China.
Bioresource technology
|October 19, 2025
概括
连续流反应堆 (CFBR) 在废水处理中更好地丰富完整的氨氧化 (comammox) 细菌. 与动态测序批量反应堆相比,CFBR中的稳定寡质条件提高了comammox丰富度和氨去除效率.
科学领域:
- 环境微生物学 环境微生物学
- 水处理工程水处理工程
- 生物地质化学生物地质化学
背景情况:
- 完全氧化氨 (comammox) 细菌在废水中提供有效的去除.
- 在寡质条件下丰富comammox细菌是低能耗治疗的关键.
- 动态与稳定的寡度营养环境对comammox丰富的影响尚不清楚.
研究的目的:
- 为了比较动态 (SBBR) 和稳定 (CFBR) 寡度营养环境对comammox细菌丰富的有效性.
- 调查反应堆类型对comammox丰富度,活动和社区结构的影响.
- 评估丰富的comammox细菌的基质亲和力和代谢基因表达.
主要方法:
- 使用测序批量生物膜反应器 (SBBR) 进行动态和连续流动生物膜反应器 (CFBR) 稳定寡度条件下.
- 使用16S rRNA基因测序和amoA基因拷贝数量的量化comammox细菌的丰富性.
- 进行了基质亲和度测试并分析了代谢基因表达 (amoABC).
- 通过网络分析研究微生物社区结构和生态相互作用.
主要成果:
- 与SBBR相比,CFBR操作显著增加了Nitrospirota的相对丰度 (0.21%至10.28%) 和amoA基因拷贝数 (1.39 × 10^5拷贝/毫克生物量).
- 遗传学分析显示,Ca.占主导地位. 在CFBR中含有类似酸盐和酸盐的Nitrospiracomammox.
- 在CFBR中丰富的微生物表现出更高的氨亲和力 (KNH4+-N = 4.967 ± 1.771 mg-N/L) 和稳定的氨去除.
- CFBRs促进了amoABC和代谢基因的上调;SBBRs显示出更密集的模块化微生物网络.
结论:
- 连续流生物膜反应器 (CFBR) 对于丰富comammox细菌比测序批量生物膜反应器 (SBBR) 更有效.
- 在CFBRs中稳定的寡性条件增强了comammox增殖,基质亲和力和代谢基因表达.
- 在CFBR中的comammox细菌采用了特定的生态策略,与有机碳代谢类人群相互作用,以增强适应性.
相关概念视频
Metabolism of Chemolithotrophs
760
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
760
Inorganic Nitrogen Assimilation
457
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
457


