Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The role of human milk oligosaccharides in shaping and restoring infant gut microbiota: population-based cohort study.

The American journal of clinical nutrition·2026
Same author

Evaluation of ensilication technology for ambient DNA preservation.

NAR molecular medicine·2026
Same author

Prenatal Isolated Congenital Diaphragmatic Hernia: A Rare Clinical Presentation of a <i>GATA4</i> Pathogenic Variant.

Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·2025
Same author

Advancing recombinant antibody production in E. coli: Optimization of expression and purification via dual GFP promoter and imaging technology.

Protein expression and purification·2025
Same author

Glyphosate and phosphate treatments in soil differentially affect crop microbiomes depending on species, tissue and growth stage.

Scientific reports·2025
Same author

Evaluation of Bridge Capture technology for mutation profiling in liquid biopsies of metastatic colorectal cancer patients.

Scientific reports·2025

相关实验视频

Updated: Jan 14, 2026

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

2.0K

桥梁捕获允许经济高效,快速和敏感的分子精确诊断.

Simona Adamusová1, Anttoni Korkiakoski1, Nea Laine2

  • 1Genomill Health Inc., Turku, Finland; Department of Biology, University of Turku, Turku, Finland.

The Journal of molecular diagnostics : JMD
|October 19, 2025
PubMed
概括

桥捕获是一种新的液体活检技术,用于检测癌症突变. 它提供了高灵敏度,成本效益和可扩展性,改进了循环瘤DNA分析的现有方法.

更多相关视频

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

1.4K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.8K

相关实验视频

Last Updated: Jan 14, 2026

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

2.0K
Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

1.4K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.8K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 癌症的诊断 癌症的诊断

背景情况:

  • 通过向下一代测序 (NGS) 分析循环瘤DNA (ctDNA) 的液体活检越来越多地被使用.
  • 现有的图书馆准备方法在可扩展性,成本,灵敏度和面板大小之间存在权衡.

研究的目的:

  • 介绍和评估Bridge Capture,一种新的液体活检技术.
  • 为了比较Bridge Capture的性能与已建立的方法来检测变异型等位基因频率 (VAF).

主要方法:

  • 对24个模仿ctDNA的人工结直肠生物样本的分析.
  • 桥梁捕获与Archer LIQUIDPlex和AmpliSeq热力发电系统版本2的Illumina的比较.
  • 测序深度,实验室间可重复性,自动化兼容性和面板可扩展性的评估.

主要成果:

  • 桥捕获检测到最低的VAFs,显示与阿切尔液态复合 (R2 = 0.995) 和AmpliSeq CHPv2 (R2 = 0.988) 的强烈相关性.
  • 证明了高的实验室间可重复性和自动化和手动工作流程之间的一致性.
  • 实现了300%的面板尺寸增加,对性能和交叉反应能力的影响微不足道,这表明了高的多重复合能力.

结论:

  • 桥捕获为ctDNA分析提供了一种成本高效,灵敏和可扩展的解决方案.
  • 该技术与标准NGS平台兼容,并优化了测序能力.
  • 桥梁捕获有潜力通过改进的诊断显著提升癌症突变检测.