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从S. cerevisiae中生产和净化人类SMC5/6核心复合体

Hemanta Adhikary1, Damien D'Amours2

  • 1Department of Cellular and Molecular Medicine, Ottawa Institute of System Biology, University of Ottawa, Ottawa, ON, Canada.

Methods in molecular biology (Clifton, N.J.)
|October 19, 2025
PubMed
概括

研究人员开发了一种基于酵母的方法来生产和净化人类SMC5/6复合体,这对基因组稳定至关重要. 这一突破使得进一步对这种必不可少的酶进行结构和功能研究成为可能.

关键词:
亲和色谱法是一种亲和色谱法.人类SMC5/6复合体微生物细胞工厂是一个微生物细胞工厂.蛋白质净化 蛋白质的净化再组合蛋白质的表达方式这种植物是Saccharomyces cerevisiae.

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