相关实验视频

Updated: Jan 14, 2026

Rat Liver Perfusion and Primary Hepatocytes Isolation: An Old Procedure Crucial for Cutting-Edge 3D Organoids Culture

Rat Liver Perfusion and Primary Hepatocytes Isolation: An Old Procedure Crucial for Cutting-Edge 3D Organoids Culture

Published on: November 22, 2024

在二维培养中肝细胞脱差显示出广泛的转录和蛋白质组重新连接

Morten Dall¹, Ben Stocks, Daniel T Cervone

¹Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark.

Hepatology communications

|October 20, 2025

概括

主要肝细胞在二维培养过程中经历了显著的转录组和蛋白质组变化,影响了它们对肝脏代谢研究的有用性. 这项研究突出了培养肝细胞中基因和蛋白质表达的改变.

科学领域:

*肝细胞生物学和肝脏研究.
* 转录组学和蛋白组学.
*体外建模和细胞培养.

背景情况:

* 初级肝细胞对于研究肝脏代谢的体外模型至关重要.
* 长时间培养肝细胞导致脱差,限制了模型的适用性.
* 了解培养肝细胞中的细胞变化对于准确的研究至关重要.

研究的目的:

*为了全面描述2D培养后初级肝细胞的转录组和蛋白质组变化.
* 评估短期培养对肝细胞基因和蛋白质表达特征的影响.
* 评估培养肝细胞在模拟肝脏生理学的有用性.

主要方法:

*新鲜分离和二维培养的初级肝细胞的转录组和蛋白质组分析.
* 活体肝脏组织与体外培养细胞之间的基因和蛋白质表达的比较.
*对1万多个基因和3000多个蛋白质进行了定量分析.

主要成果:

*与新鲜细胞相比,24小时的二维培养显著改变了超过1万个基因和3000个蛋白质.
*培养减少了转录异质性,并导致肝脏区域标记物的丧失.
*观察到的变化包括细胞外矩阵蛋白,线粒体/核糖体蛋白和急性相反应蛋白.

结论:

*初级肝细胞培养显著重组转录组和蛋白质组,可能会影响肝脏研究的实用性.
* 开发了"Hepamorphosis"闪亮的应用程序,用于探索肝细胞中的RNA/蛋白质相关性和区分.
* 该应用程序促进了细胞类型特定转录在全肝和培养肝细胞的分析.

关键词:

在RNAseqqq中使用.没有分化,没有分化.肝细胞肝细胞肝细胞肝脏肝脏肝脏肝脏肝脏肝脏多种主题的多种主题.蛋白质组学蛋白质组学一个核的RNAseqqq.

更多相关视频

Human Liver Spheroids from Peripheral Blood for Liver Disease Studies

Human Liver Spheroids from Peripheral Blood for Liver Disease Studies

Published on: January 27, 2023

Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue

Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue

Published on: May 5, 2022

相关概念视频

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...

Forced Transdifferentiation

Forced Transdifferentiation

Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...