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相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

8.8K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
14.8K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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相关实验视频

Updated: Jan 14, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
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在皮肤上,启用微镜头的摄像头模块用于光斑对比光学光谱/断层扫描.

Andres Quiroga1, Lorenzo Cortese1, Manish Verma1

  • 1ICFO - Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels (Barcelona), Spain.

Biomedical optics express
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概括

我们开发了一个紧的斑点对比光学光谱/断层扫描 (SCOS/SCOT) 系统,使用微型镜头直接与皮肤接触. 这个系统准确地跟踪肌肉血液流动和脉动动态,为可穿戴的SCOS/SCOT设备铺平了道路.

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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
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科学领域:

  • 生物医学光学 生物医学光学
  • 医疗成像医学成像
  • 光学光谱学是指光学光谱学.

背景情况:

  • 斑点对比光学光谱/断层扫描 (SCOS/SCOT) 对非侵入性血流监测非常有价值.
  • 现有的SCOS/SCOT系统通常需要光纤合,这限制了便携性和易用性.
  • 开发紧,可穿戴的SCOS/SCOT设备对于持续的生理监测至关重要.

研究的目的:

  • 为了引入和验证一个新的,紧的SCOS/SCOT系统,使用集成的微镜头阵列.
  • 评估系统对直接皮肤接触测量和同时多距离采集的性能.
  • 为了证明系统在体内监测肌肉血流和脉动动态的能力.

主要方法:

  • 设计了一种集成成像SCOS/SCOT配置,在CMOS摄像头上设有113个微镜头,从而消除了光纤合.
  • 该系统在使用幻象和在人前臂肌肉上进行了活体验证.
  • 测量是在多个源探测器分离点和相机曝光时间进行的.

主要成果:

  • 该系统与对应-扩散模型在ex vivo和in vivo测量方面表现出极好的一致性.
  • 在体内数据显示高信号与噪声比率跟踪脉动性血流 (检测>4心脏波).
  • 该系统对肌肉血流的微小变化表现出敏感性.

结论:

  • 基于微镜头阵列的SCOS/SCOT系统可以直接与皮肤接触,同时进行多距离测量.
  • 这种紧的设计克服了可穿戴SCOS/SCOT设备开发的关键挑战.
  • 经过验证的系统显示了先进,非侵入性肌肉血流监测的前景.