紫外线可固化树脂作为STORM的快速和优质密封剂
Youngseop Lee1, Minchol Lee1, Yoonhee Park1
1Department of Applied Bioengineering, Seoul National University, Seoul 08826, Republic of Korea.
Biomedical optics express
|October 20, 2025
概括
紫外线 (UV) 可固化树脂为静态光学重建显微镜 (STORM) 成像缓冲器提供了有效的,气密的密封. 这种新型密封剂显著延长成像持续时间,并提高超分辨率显微镜的工作流程效率.
科学领域:
- 生物物理学的生物物理.
- 显微镜技术的使用方法
- 光学成像技术的使用.
背景情况:
- 随机光学重建显微镜 (STORM) 需要具有光交换激活器和氧吸收器的专用缓冲器.
- 葡萄糖氧化酶和催化酶 (GLOX) 系统是常见的,但会导致缓冲区酸化,限制成像时间.
- 缓冲器pH稳定性和有效密封对于长时间的STORM成像至关重要.
研究的目的:
- 引入和评估紫外线 (UV) 可固树脂作为STORM成像缓冲器的新型密封剂.
- 为了比较UV树脂与STORM应用的传统密封剂的性能.
- 评估紫外线树脂密封对缓冲器寿命和图像质量的影响.
主要方法:
- 紫外线可固化树脂的应用作为基于GLOX的STORM缓冲器的密封剂.
- 紫外线树脂与指甲油,CytoSeal 60和5分钟环氧的比较.
- 在COS-7细胞中使用Alexa Fluor 647和CF568染料对单分子闪和图像质量指标的评估.
主要成果:
- 紫外线树脂在几秒钟内提供快速,密封的密封.
- 紫外线树脂在保护缓冲器完整性和成像质量方面优于传统密封剂.
- 关键的单分子和图像质量指标在UV树脂密封后至少维持了48小时.
结论:
- 紫外线可固化树脂是STORM显微镜的实用,有效和廉价密封剂.
- 紫外线树脂显著延长了STORM成像持续时间,并增强了实验工作流程.
- 这种新的密封策略解决了与传统方法和缓冲区酸化相关的局限性.
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