Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.2K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.2K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

20.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
20.0K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.0K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Lenvatinib Combined with New FP Hepatic Arterial Infusion Chemotherapy for Unresectable Hepatocellular Carcinoma: Clinical Efficacy, Vascular Remodeling, and Implications for Immuno-Oncology-Systemic Combination Therapy.

Current oncology (Toronto, Ont.)·2026
Same author

Aplastic or twig-like middle cerebral artery with the RNF213 variant: illustrative cases.

Journal of neurosurgery. Case lessons·2026
Same author

Altitude-dependent variations in environmental conditions and human activities regulate microbial community assembly and carbon metabolism patterns in headwater rivers.

Environmental research·2026
Same author

Delayed surgical management of extensive bilateral multilobar congenital pulmonary airway malformation.

BMJ case reports·2026
Same author

Distinct postsynaptic morphogenetic strategies across <i>Drosophila</i> embryonic muscles during neuromuscular junction formation.

bioRxiv : the preprint server for biology·2026
Same author

Genomic profiling of meiotic errors and early malignant transformation events in ovarian mature teratoma.

Reproduction (Cambridge, England)·2026
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
查看所有相关文章

相关实验视频

Updated: Jan 14, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.1K

具有rsEGFP2的二维非线性结构化照明显微镜.

Shaoheng Li1, Ryo Tamura2,3, Kota Banzai2,4

  • 1School of Electrical and Computer Engineering, University of Georgia, Athens, GA, USA.

Biomedical optics express
|October 20, 2025
PubMed
概括
此摘要是机器生成的。

图形消耗非线性结构化照明显微镜 (PD-NSIM) 使用rsEGFP2光蛋白在活细胞成像中实现80nm以下的分辨率. 这种技术为先进的生物成像提供了速度,分辨率和寿命的有希望的组合.

更多相关视频

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

2.0K
Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

8.0K

相关实验视频

Last Updated: Jan 14, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.1K
Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

2.0K
Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

8.0K

科学领域:

  • 生物物理学的生物物理.
  • 细胞生物学 细胞生物学
  • 显微镜的使用方法

背景情况:

  • 非线性结构化照明显微镜 (NSIM) 提供高分辨率,但其已发表的应用有限.
  • 现有的NSIM技术在速度,分辨率和光体寿命方面面临挑战.

研究的目的:

  • 通过使用光蛋白rsEGFP2.2.来证明NSIM (PD-NSIM) 的模式性耗尽.
  • 为了实现高分辨率的活细胞成像,提高速度和寿命.

主要方法:

  • 使用快切换光蛋白rsEGFP2.2的利用型式耗尽NSIM (PD-NSIM).
  • 在U2OS细胞中实时2D成像了actin纤维.
  • 分析了实空间和里埃空间的图像分辨率.

主要成果:

  • 在活细胞成像中达到80nm以下的分辨率.
  • 证明了rsEGFP2作为PD-NSIM的光体的有效性.
  • 展示了使用rsEGFP2.2的第一个现场2D PD-NSIM应用程序.

结论:

  • 与rsEGFP2一起的PD-NSIM是高分辨率活细胞成像的可行方法.
  • rsEGFP2为NSIM提供了速度,分辨率和寿命的宝贵组合.
  • 这种技术提升了超分辨率显微镜在生物研究中的功能.