与G蛋白结合的雌激素受体调节介酶干细胞机械传导和分化
Hao Wang1,2, Ofra Ben Menachem-Zidon3, Ashish Pandey1,2
1Tech4Health Institute, NYU Langone Health, New York, NY, USA.
Cellular and molecular bioengineering
|October 20, 2025
概括
G蛋白结合雌激素受体 (GPER) 激活调节介质干细胞 (MSC) 的机制传导和分化. GPER促进骨质生成并抑制脂肪生成,影响细胞迁移和细胞骨组织.
科学领域:
- 生物化学 生化学
- 细胞生物学 细胞生物学
- 生物医学工程 生物医学工程
背景情况:
- G蛋白结合雌激素受体 (GPER) 是细胞过程的关键调节者.
- 在机械转导和干细胞分化的GPER的作用是复杂的和辩论.
- 介质干细胞 (MSC) 对于组织再生至关重要,它们的分化受到机械线索的影响.
研究的目的:
- 研究GPER激活对MSC机械传导和分化的特定影响.
- 阐明GPER信号与细胞外矩阵 (ECM) 线索在调节MSC行为中的相互作用.
- 了解GPER对MSC命运决定的影响的机制.
主要方法:
- 用GPER激动剂 (G1) 治疗MSC,并在纤维内素涂层表面和微模式上培养.
- 细胞迁移被评估使用化学动力学和化学试验测试.
- 使用特定的染色方法 (性酸酶和油红色O) 量化MSC骨质生成和脂肪生成.
主要成果:
- GPER激活改变了细胞骨组织,减少了细胞极化和应激纤维,并减少了RhoA信号传递.
- 激活GPER可以降低MSC迁移速度.
- 在MSC中,GPER显著促进骨质生成和抑制脂肪生成,在微型表面上观察到复杂的相互作用.
结论:
- GPER直接调解MSC机械传导,部分通过RhoA无活化.
- 持续的GPER激活促进骨质生成,并抑制脂肪生成,独立于减少的细胞极化和张力.
- 研究结果强调了GPER在MSC分化中的作用及其与ECM的相互作用,这表明GPER在再生医学和激素疗法中的治疗潜力.
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