评估医院废水处理对细菌组成和抗菌素耐药性的影响
Yan Meng1, Yi Xu2,3,4, Dongping Hu2,3,4
1Department of Clinical Laboratory, Zhejiang Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Frontiers in microbiology
|October 20, 2025
概括
医院废水处理系统显著改变细菌群落,减少抗菌素耐药性基因 (ARG). 然而,一些ARG,特别是那些在等离子体上,仍然存在,这强调了需要先进的治疗策略.
科学领域:
- 环境微生物学 环境微生物学
- 公共卫生 公共卫生
- 废水工程 废水工程
背景情况:
- 医院废水处理系统 (HWTS) 对于控制抗菌素耐药基因 (ARG) 和抗生素耐药细菌的传播至关重要.
- 了解HWTS对细菌组成和ARG丰度的影响对于公共卫生至关重要.
研究的目的:
- 评估HWTS如何影响细菌社区结构.
- 评估治疗后医院废水中ARG相对丰度的变化.
- 调查ARG在治疗系统中的移动性潜力.
主要方法:
- 废水样本从一所大学医院的HWTS的进水和废水中收集.
- 使用元基因组测序来分析细菌组成和ARG概况.
- 遗传学分析和开放式读取碎片分析被用来识别细菌和ARG.
主要成果:
- HWTS改变了细菌组成,减少了Gammaproteobacteria,但显示了Mycobacterium和Zoogloea的密切基因关系.
- 总共有140个非冗余ARG被确定,其中β-乳酸和氨基糖化物耐药性基因是最普遍的.
- 总的来说,ARG的相对丰度显著下降 (p < 0.0001),70%的最后抗生素耐药性基因变得无法检测. 然而,四级化合物耐药性基因增加.
结论:
- HWTS有效地减少了许多ARG的丰富性,包括那些作为最后手段的抗生素.
- 位于等离子体上的ARG和邻近移动基因元素的ARG显示移除效率降低,这表明水平基因转移的潜力.
- 有效的废水处理对于缓解抗菌素耐药性的环境传播至关重要.
关键词:
抗微生物耐药性基因是抗微生物耐药性的基因.医院的废水处理方法基因基因组测序 (metagenomic sequencing) 是一种对基因基因基因的测序.移动的遗传元素是移动的.废水处理的有效性 废水处理的有效性更多相关视频
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