在线监测基于芯片的微型 perfusion 发酵
Sabrina M Cramer1,2, Shubham Gurav1,3, David Glinsner1,3
1acib - austrian centre of industrial biotechnology, Muthgasse 11, Vienna 1190, Austria.
ACS omega
|October 20, 2025
概括
一个新的在线分析平台可以实时监测微生物反应器发酵. 该系统允许有效选和开发生物工艺,提高菌株生产率和产品产量.
科学领域:
- 生物技术是生物技术.
- 分析化学 分析化学
- 生物工艺工程 生物工艺工程
背景情况:
- 在生物制造中,传统的离线采样对发酵参数提供了有限的反.
- 基准生物工艺的开发需要改进实时监控的方法.
研究的目的:
- 开发一个敏感和选择性的在线分析平台,用于监测基于芯片的微生物反应器.
- 为了实现发酵生物过程参数和代谢物的实时分析.
主要方法:
- 使用商用组件构建了一个平台:门,2DLC硬件,LC分离和在线联质谱.
- 培养了*Saccharomyces cerevisiae*的微生物反应器 (20微升),具有低流量 perfusion 速率.
- 采用了多重配置的流选择和适应微尺度的2DLC-MS/MS系统.
主要成果:
- 该平台成功地在5-24小时内监测了生产乳酸的S. cerevisiae的发酵过程.
- 在线测量并行发酵中的代谢物,没有阻塞或交叉污染.
- 该系统证明适合在线监测产品数量和关键代谢物.
结论:
- 开发的平台为发酵生物技术提供实时监测,使得快速的菌株选择和选成为可能.
- 生物材料和媒介的最小消耗 (<1.5毫升/24小时) 使该平台具有高效率.
- 可适应的平台可用于监测各种生物分子,并针对其他生物技术产品.
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