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相关概念视频

Epistasis01:39

Epistasis

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In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
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Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
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Conservation of Protein Domains Over Different Proteins02:26

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Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
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Mass Spectrometry: Isotope Effect01:13

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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
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Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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相关实验视频

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SWATH-MS蛋白质组学数据显示,在正常牛肉和黑色牛肉之间,蛋白质的丰富程度不同.

Laura González-Blanco1, Mamen Oliván1, Yolanda Diñeiro1

  • 1Área de Sistemas de Producción Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA#@SERIDA_info), Ctra. AS-267, PK 19, 33300 Villaviciosa, Spain.

Data in brief
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概括

使用SWATH-MS蛋白质组学分析了深色,坚硬和干燥 (DFD) 牛肉,这是一个昂贵的质量缺陷. 研究人员确定了35种蛋白质,这些蛋白质可以作为预测DFD牛肉的生物标志物,有助于早期检测和质量改善.

关键词:
牛肉质量缺陷 牛肉质量缺陷牛的肌肉肌肉是什么?高肌肉pH的牛肉肉.肌肉蛋白质组是一种肌肉蛋白质组.进行了死后解剖.蛋白质生物标志物 蛋白质生物标志物

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科学领域:

  • 食品科学 食品科学 食品科学
  • 蛋白质组学是指蛋白质组学.
  • 动物科学 动物科学

背景情况:

  • 黑色,坚硬和干燥的 (DFD) 牛肉会造成重大经济损失,并由于其不良的外观和增加的腐败,导致消费者拒绝.
  • 牛肉DFD的潜在机制是复杂的和多因素的,屠宰前的因素起作用,但不能完全阐明这种情况.
  • 蛋白质组方法对于了解DFD牛肉的分子基础和识别潜在生物标志物是有价值的.

研究的目的:

  • 用SWATH-MS蛋白质学来描述和量化DFD牛肉中的死后肌肉蛋白质.
  • 为了确定与DFD牛肉状况相关的蛋白质表达差异.
  • 发现候选蛋白质生物标志物用于早期预测和更好地表征DFD牛肉.

主要方法:

  • SWATH-MS (所有理论质谱的顺序窗口获取) 蛋白质组学被用于深入的蛋白质组分析.
  • 分析了一个全面的数据集,包括26个DFD牛肉样本 (pH24 ≥6.2) 和26个对照样本 (5.4 ≤ pH24 ≤ 5.6).
  • 来自阿苏拉尼亚德洛斯瓦莱斯 (Asturan de los Valles) 一岁公牛的长胸和腰部肌肉样本是在死后24小时收集的.

主要成果:

  • 在肌肉样本中,总共量化了735种蛋白质.
  • 在DFD和对照样本之间观察到蛋白质丰度的显著差异.
  • 35种蛋白质的丰度明显不同,这表明它们可能成为DFD牛肉的生物标志物.

结论:

  • 该研究提供了DFD牛肉的全面蛋白质组数据集,促进了进一步的研究.
  • 已识别的35种蛋白质代表了早期预测DFD牛肉的潜在生物标志物.
  • 这项研究为完善DFD牛肉生物标记物选择和理解潜在的生物机制的整合经济学方法做出了贡献.