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基因组缩小的Pseudomonas putida在氧气耗尽后超过了野生型菌株的竞争力

Jesper W Jensen¹, Pablo I Nikel², John M Woodley¹

¹Department of Chemical and Biochemical Engineering Technical University of Denmark (DTU) Lyngby Denmark.

Engineering in life sciences

|October 20, 2025

概括

一种基因组缩小的Pseudomonas putida (SEM10) 菌株在低氧条件下保持生长并超越野生类型. 这种精简的菌株对面临氧气限制的工业生物工艺充满希望.

科学领域:

微生物生物技术微生物生物技术
合成生物学合成生物学
代谢工程是代谢工程.

背景情况:

工业生物工艺依赖于强大的微生物菌株,如Pseudomonas putida.
氧气的可用性是大型有氧发酵的关键因素,往往导致梯度和限制.
基因组缩小旨在通过删除非必不可少的基因来提高细胞效率,从而有可能改善资源分配.

研究的目的:

评估基因组缩小的Pseudomonas putida菌株 (SEM10) 在不同氧气部分压力 (pO2) 下的生长特征.
在氧气有限的条件下,将SEM10与野生型菌株 (KT2440) 的性能进行比较.
评估SEM10作为一个工业生物工艺平台的潜力,在那里氧气稀缺是一个问题.

主要方法:

野生类型的P. putida KT2440和基因组缩小株SEM10在批量模式下进行培养.
在通风气中操纵氧的部分压力 (pO2),以模拟不同的氧气可用性.
在非限制条件和氧气限制条件下测量生物质产量和最大特异增长率.

主要成果:

菌株SEM10在非DO有限生长期间,在葡萄糖上的生物质产量比野生类型高12.7%.
在氧气耗尽 (pO2 = 0.0525 atm) 的情况下,与非限制条件相比,SEM10保持了相似的生物质产量和生长率.
在氧气限制下,SEM10显著超过KT2440,分别获得了23.3%和35.5%更高的葡萄糖和氧气生物质产量.

结论:

在P. putida中减少基因组不会对整体生长特性产生不利影响,并且可以提高效率.
基因组缩小的SEM10菌株在缺氧条件下表现出优越的耐受性和竞争能力.
精简的P. putida菌株,如SEM10,为强大的工业生物工艺提供了一个有希望的平台,特别是那些容易受到氧气限制的生物工艺.

关键词:

DO限制限制的限制.伪omonas putida可以使用.基因组缩小 - 基因组缩小增长的特征增长的特征微生物培养的微生物培养

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