聚乙烯纳米塑料的细胞毒性涉及太平洋的血细胞中的线粒体功能障碍和DNA损伤
Larissa Cristine de Carvalho Penha1, Thuanne Braúlio Hennig2, Diego José Nogueira3
1Laboratory of Cellular Defenses, Department of Biochemistry, Federal University of Santa Catarina, 88037-000, Florianópolis, Brazil.
Ecotoxicology and environmental safety
|October 20, 2025
概括
聚乙烯纳米塑料通过破坏DNA和增加氧化应激,主要通过线粒体破坏,损害太平洋血细胞. 这凸显了有氧代谢在纳米塑料毒性评估中的重要性.
科学领域:
- 环境毒理学环境毒理学
- 海洋生物学 海洋生物学
- 细胞生物学 细胞生物学
背景情况:
- 纳米塑料对海洋生态系统构成越来越大的威胁,影响免疫功能,氧化应激和能量代谢.
- 聚乙烯纳米塑料 (NanoPS) 是普遍存在的环境污染物.
研究的目的:
- 为了研究纳米PS暴露对太平洋血细胞的细胞效应.
- 为了在体外确定NanoPS的分布,代谢中断和基因毒性.
- 阐明线粒体代谢在纳米塑料毒性的作用.
主要方法:
- 在体外细胞生物测试使用太平洋 (Crassostrea gigas) 血细胞.
- 传输电子显微镜用于NanoPS分布分析.
- 雷萨苏林和中性红色检测细胞毒性.
- 测量活性氧物种和DNA损伤.
- 使用银河糖和葡萄糖补充剂进行代谢操纵.
主要成果:
- 纳米PS被血细胞内化,在囊泡,细胞质和细胞核中积累.
- 代谢活动对NanoPS比溶酶体完整性更敏感.
- 纳米PS暴露增加了活性氧物种和DNA损伤,即使在低度.
- Galactose 补充剂加剧了 NanoPS 毒性,而葡萄糖补充剂减少了它.
- 线粒体解器FCCP增加了DNA损伤,但没有影响生命力.
结论:
- 线粒体是双细胞中纳米塑料毒性的敏感目标.
- 有氧代谢在介导纳米塑料诱导的细胞损伤方面发挥着至关重要的作用.
- 在体外基于血细胞的测试是评估海洋生物中纳米塑料毒性机制的宝贵工具.
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