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针对ASFV编码的早期蛋白质pI73RR的单克隆抗体的开发和表征

Yurun Miao¹, Pu Zhao², Zhenzhong Wang¹

¹The Sanya Institute of Nanjing Agricultural University, Laboratory of Emerging Animal Diseases and One Health, Nanjing Agricultural University, Nanjing, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China; China Animal Health and Epidemiology Center, Qingdao, China.

International journal of biological macromolecules

|October 20, 2025

概括

研究人员开发了针对非洲猪瘟病毒 (ASFV) pI73R蛋白的单克隆抗体. 这些抗体显示出高反应性和效率,为ASFV诊断和疫苗开发提供了潜力.

科学领域:

兽医病毒学兽医病毒学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

非洲猪瘟病毒 (ASFV) 是一种致命的病原体.
病毒蛋白pI73R在感染初期得到表达,并且具有高度的抗原性.
pI73R是ASFV诊断和疫苗的潜在目标.

研究的目的:

开发和描述针对ASFV pI73R蛋白的单克隆抗体 (mAbs).
评估这些mAbs用于ASFV检测的诊断潜力.
调查mAbs识别的表位和它们的功能活动.

主要方法:

在大肠杆菌中ASFV pI73R蛋白的表达.
使用混合体技术生产单克隆抗体 (mAbs).
使用西部斑 (WB),免疫沉 (IP) 和间接免疫光 (IFA) 验证mAbs.
使用截断的蛋白质进行皮层图谱绘制.
阻断ELISA和中和测试试验.

主要成果:

建立了两个稳定的混合瘤细胞系 (5D5和12F2),分泌抗pI73R mAbs.
两种mAbs在WB,IP和IFA试验中都显示出对ASFV的反应性.
mAbs 特别识别了在 pI73R.上保存的线性表位 LTAKNIKVVI 30.
一个mAb (pI73R-5D5) 显示出对ASFV感染的阻断活性有限.

结论:

针对ASFV pI73R的单克隆抗体具有很高的反应性和检测效率.
这些mAbs对ASFV诊断和潜在的疫苗开发充满希望.
需要对pI73R功能和mAb应用进行进一步的研究.

关键词:

在ASFV中,ASFV是ASFV.在地图上绘制出地图的地图.单克隆抗体是一种单克隆抗体.pI73RR 在线阅读

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