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3D4细胞表现出与膜巨细胞身份不一致的转录特征

Wenjuan Ma¹, Frieder Hadlich¹, Nares Trakooljul¹

¹Working Group Physiological Genomics, Research Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.

Veterinary research

|October 20, 2025

概括

广泛使用的3D4细胞系,被认为是猪膜巨细胞模型,对免疫刺激没有反应. 转录组分析显示,它们被错误分类,并具有呼吸上皮质特征,而不是髓质特征.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
基因组学就是基因组学.

背景情况:

3D4细胞系在免疫学研究中通常被用作猪膜巨模型.
初步观察表明,3D4/21细胞对脂聚糖刺激缺乏反应,这促使进一步调查.

研究的目的:

为了研究3D4/21和3D4/2细胞系对托尔类受体 (TLR) 配体的免疫反应.
通过比较3D4/21细胞的转录组形状与初级猪膜巨细胞 (PAM) 来澄清观察到的缺乏反应.

主要方法:

挑战3D4/21和3D4/2细胞系与各种TLR4和TLR2/1连接体.
从小猪中分离初级PAM进行比较分析.
对非刺激和刺激的3D4/21细胞进行转录组分析 (RNA测序) 与PAM.

主要成果:

3D4细胞系未能对TLR连接体产生促炎反应 (IL1B,TNF表达).
在3D4/21细胞和PAM细胞之间发现了广泛的转录组差异,其中有10,718个差异表达的基因.
关键的巨细胞标记物 (SPI1,IRF8) 和病原体识别基因 (TLR,NOD2) 在3D4/21细胞中显著下调或不存在,而上皮细胞标记物高度表达.

结论:

3D4细胞系被错误分类,不代表有功能的猪膜巨细胞.
这些细胞系表现出呼吸道上皮细胞的特征,而不是髓状细胞系细胞.
这些发现需要重新评估3D4细胞系在免疫学研究中的使用情况,并强调它们作为上皮细胞模型的潜力.

关键词:

3D4 细胞系 3D4 细胞系猪的膜巨细胞.收费类受体的收费类受体免疫代谢的免疫代谢.脂多糖类糖化物 (Lipopolysaccharide) 是一种脂多糖类糖.转录组 (transcriptome) 是一个转录组.

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