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Updated: Jun 18, 2026

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通过甲固定,冷保存和多重复合来增强单细胞ATAC测序,以便灵活分析

Tobias Hohl1,2, Ulrike Bönisch1, Thomas Manke1,3

  • 1Max-Planck-Institute of Immunobiology and Epigenetics, 79108, Stübeweg 51, Freiburg im Breisgau, Germany.

BMC research notes
|October 20, 2025
PubMed
概括

使用轻度甲固定和冷保存的新方法允许从存档样本中获得高质量的批量和单细胞ATAC-seq (通过测序测定转化酶可访问的染色体),克服后勤挑战.

关键词:
ATAC-seqq 的使用情况.批量效应是一种批量效应.结冷保存是一种冷保存.形式甲固定的形式甲多重复合是一种多重复合.样品保存 样品的保存一个单细胞分析.

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RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...