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一个基于CFD的数字框架,用于轨道摇摆生物反应器的扩展优化

Soo Hyun Ryu¹, Young Jin Kim¹, Jae Hong Jeon¹

¹Division of Biological Science and Technology, Yonsei University, 1 Yonseidae-gil, Wonju, Gangwon-do, Republic of Korea.

Biotechnology journal

|October 21, 2025

概括

使用CFD和RMSE分析的新数字框架优化了轨道摇摆生物反应器的扩展. 这确保了在一次性系统中保持一致的细胞培养性能.

科学领域:

生物技术是生物技术.
化学工程是化学工程的重要组成部分.
过程开发过程开发

背景情况:

生物反应器扩大规模对于持续的细胞培养性能至关重要.
跨尺度的水力动力学相似性对于强大的生物过程至关重要.
轨道摇摆生物反应器提供了独特的优势,但需要优化扩展战略.

研究的目的:

开发和验证计算流体动力学 (CFD) 引导的数字框架,以优化CELBIC轨道摇摆生物反应器的扩展.
为了确保CELBIC5和CELBIC50系统之间的水力动力学相似性.
在一次性生物反应器中建立数字过程开发的系统战略.

主要方法:

使用基于Boltzmann的格子CFD模拟来评估水力动力学参数 (速度,剪切应力,能量消耗率).
开发了一种基于根平均平方误差 (RMSE) 的方法来量化跨缩放条件的水力动力学相似性.
采用响应表面分析来完善优化的扩展参数.

主要成果:

确定了CELBIC50生物反应器相对于CELBIC5参考 (1L,6°,30rpm) 的最佳扩展条件 (10L,7°,19rpm).
优化条件显示了最低的组合RMSE,表明了实质性的水力动力学等价性.
数字框架成功实现了合理的扩展,确保了流程的一致性.

结论:

整合基于CFD和RMSE的分析,为轨道摇摆生物反应器扩展提供了合理和系统的方法.
开发的数字框架促进了单次使用生物反应器系统的高效过程开发和优化.
这种方法确保了水力动力学相似性,这对于在扩展期间保持细胞培养性能至关重要.

关键词:

生物反应器的水力动力学计算流体动力学 (CFD) 计算流体动力学轨道摇摆运动轨道摇摆运动扩大规模的升级.

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