在冷母乳中微生物殖民地数量的日间变化:一项实验研究
Deniz Yaprak1, Mina Mısırlıgil2, Belma Saygılı Karagöl1
1Division of Neonatology, Department of Pediatrics, Gülhane Training and Research Hospital, University of Health Sciences, Ankara, Türkiye.
JPEN. Journal of parenteral and enteral nutrition
|October 21, 2025
概括
6个月冷母乳显著降低了其对大肠杆菌的抗菌特性. 牛奶成分的日常变化会影响其在冷保存后的抗菌质量.
科学领域:
- 人母奶的成分和储存方式
- 微生物学 微生物学
- 婴儿营养 婴儿营养
背景情况:
- 长期冷母乳可能会损害其固有的抗菌特性.
- 评估6个月冷对母乳对大肠杆菌的抗菌作用的影响至关重要.
- 研究新鲜和冷母乳的日常变化可以优化储存协议.
研究的目的:
- 为了评估母乳在六个月的冷后对大肠杆菌的抗菌疗效.
- 探索新鲜和冷母乳抗菌性质的日间变化.
- 为优化母乳储存协议提供数据.
主要方法:
- 在2025年2月至5月期间,分析了30名母亲 (15-30天龄的婴儿) 的60个母乳样本.
- 在新鲜牛奶 (24小时内) 和在-80°C冷6个月的牛奶中对大肠杆菌进行了抗微生物活性评估.
- 使用殖民地计数量的量化细菌生长在牛奶-大肠杆菌稀释后的MacConkey agar上.
主要成果:
- 6个月后,冷母乳的抗菌素质量显著降低 (97.1%),与新鲜奶相比,大肠杆菌的数量更高 (中位数为225vs6.5).
- 白天冷样本的微生物数量比夜间样本低 (中位数为95对120;P=0.006).
- 在冷样本中,诸如初级生育,剖腹产和男性婴儿等因素与更高的夜间数量有关;孕产妇年龄和妊娠年龄与微生物数量存在关联.
结论:
- 6个月的冷保存大大降低了母乳的抗菌特性.
- 人类牛奶的抗菌活性在冷后仍然存在日常变化,受母体平价和婴儿性别等因素的影响.
- 研究结果强调,需要优化储存策略,以保持母乳的有益性质.
相关概念视频
Microbial Growth Measurement: Indirect Methods
1.3K
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
1.3K
Microbial Growth Measurement: Direct Methods
1.5K
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
1.5K


