在α-蛋白质细菌中,nodD类的lysR型转录调节者的模块化进化和调控多样化
Nelly Koech1, John Muoma1, Aparna Banerjee2
1Department of Biological Sciences, School of Natural Science, Masinde MuliroUniversity of Science & Technology, P.O. Box 190, Kakamega, 50100, Kenya.
Archives of microbiology
|October 21, 2025
概括
对于树枝生物共生至关重要的nodD基因具有古老的起源和多样化的进化路径,涉及基因重复和水平基因转移. 它的调节功能超越了共生,影响细菌中的各种细胞过程.
科学领域:
- 微生物学 微生物学
- 进化生物学 进化生物学
- 基因组学就是基因组学.
背景情况:
- 节点D基因对于树枝生物中的共生结节是必不可少的.
- 它的进化历史,结构适应性和超越共生的监管范围尚未完全理解.
研究的目的:
- 研究NodD及其同类在α-蛋白质细菌中的进化起源,基因组组织和功能多样化.
- 探索节点D类系统的监管网络和目标.
主要方法:
- 基于orthogroup的泛基因组聚类和植物遗传学分析来重建NodD进化.
- 基因邻居和操作分析以评估基因组背景.
- 使用AlphaFold和PyMOL进行结构比较.
- 动机发现和全基因组扫描用于目标识别.
主要成果:
- nodD起源于古代的LysR型转录调节器 (LTTRs),经历了广泛的重复和水平基因转移 (HGT).
- 节点D的基因组架构有所变化,在根茎菌中显示保存的合成,但在自由生活的细菌中显示出分歧.
- 结构分析揭示了保存的LTTR折叠与特定物种的变化.
- 确定了参与新陈代谢,应激反应和其他细胞功能的nodD类调节者的新目标.
结论:
- nodD是一个结构保守但功能灵活的调节器,具有古老的起源.
- 它的进化是由基因重复,HGT和基因组背景塑造的,扩展其作用超出了共生.
- 节点D类系统扩大了细菌LTTRs的监管范围.
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