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鉴定,真菌毒素生成能力和生物合成基因的Alternaria spp. 来自中国的果

Yinping Li¹, Yupeng Cai², Kunmiao Yu¹

¹Institute of Pomology, Chinese Academy of Agricultural Sciences, 125100, Xingcheng, Liaoning Province, China.

International journal of food microbiology

|October 21, 2025

概括

这项研究确定了Alternaria alternata和A. arborescens物种综合体作为果中真菌毒素的关键生产者. 研究人员确定了负责五种主要Alternaria毒素生物合成的特定基因.

科学领域:

食品科学食品科学食品科学
菌类学菌类学是指菌类学.
遗传学遗传学是一种遗传学.

背景情况:

替代性毒素是新兴的真菌毒素,经常在果和果产品中发现.
负责产生毒素的特定Alternaria物种及其生物合成的遗传基础仍然不清楚.

研究的目的:

为了确定在中国污染果的Alternaria物种.
为了确定这些分离物的毒素生产概况.
确定参与五种关键Alternaria毒素生物合成的基因.

主要方法:

来自中国果种植地区的255个Alternaria菌株的隔离和鉴定 (形态和分子).
在UPLC-MS/MS分析中检测了五种Alternaria毒素 (alternariol,alternariol单甲基乙烯, altenuene,altertoxin I,tentoxin).
用PacBio和转录组测序来识别毒素生物合成基因.

主要成果:

251个分离物被确定为Alternaria alternata,4个被确定为A. arborescens物种复合物 (AASC).
254个分离物产生了多种毒素;Altertoxin I是最常见的,Altenuene是最常见的.
通过测序和同源对齐,确定了五种Alternaria毒素的生物合成基因.

结论:

中国果中的Alternaria alternata和AASC是Alternaria毒素的主要来源.
这项研究为管理果中Alternaria毒素污染提供了关键的遗传信息.
鉴定出来的基因为未来的研究和控制策略提供了基础.

关键词:

另一个是Alternaria.生物合成基因的基因这就是PacBio的序列.在 UPLC-MS/MS 中使用.

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