序列氧干预驱动了新型的AOB-DGAO相互作用网络,使得在同时部分化和内源性脱 (SPNED) 过程中能够进行先进的去除
Li Zhang1, Yiqing Zhang1, Yu-You Li2
1National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing, China.
Bioresource technology
|October 21, 2025
概括
这项研究引入了一种用于同时部分化和内源性脱化 (SPNED) 的新型顺序氧气策略,以加强废水处理. 该方法快速建立一个稳定的微生物网络,实现超过99%的去除效率,低碳输入.
科学领域:
- 环境生物技术和废水处理工程.
- 微生物生态学和工程系统中的功能基因组学.
背景情况:
- 同时部分化和内源性脱 (SPNED) 提供了低碳废水处理潜力.
- 挑战包括酸盐氧化细菌 (NOB) 繁殖和聚酸盐 (PHA) 枯竭在化糖原积聚生物 (DGAO) 中.
研究的目的:
- 开发和验证一个顺序的氧气干预策略,以有效地启动和运行SPNED.
- 为了阐明微生物相互作用和代谢途径驱动增强的去除.
主要方法:
- 实施一个连续的无氧/无氧和有氧运行模式.
- 应用共发生网络分析和元基因组学用于微生物群落概况.
- 代谢途径分析侧重于关键酶和细胞内代谢物 (NADH).
主要成果:
- 在46天内实现了SPNED的启动,除效率高于99%.
- DGAO丰富 (9.22%至28.25%) 抑制了NOB并支持氨氧化细菌 (AOB) 的生长 (22.69倍的增长).
- 增强的DGAO代谢 (β-氧化,TCA循环) 提高了细胞内NADH (1.76倍),维持了脱.
结论:
- 拟议的战略有效地建立了一个稳定的AOB-DGAOs网络,以有效地去除.
- DGAO表现出以前未知的NOB抑制功能,对于过程稳定至关重要.
- 这种方法可以有效地利用内源碳和先进的废水处理.
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