相关实验视频

Updated: Jan 14, 2026

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Published on: November 8, 2024

通过转化生长因子β-1信号调节胎盘细胞细胞功能

Caelan A MacPhee¹, Bryony V Natale¹, Curtis Noordhof^2,3

¹Department of Biomedical and Molecular Sciences, Queen's University, 18 Stuart St., Kingston, ON, K7L 3N6, Canada.

Scientific reports

|October 21, 2025

概括

转化生长因子β-1 (TGFβ-1) 影响胎盘细胞周功能,促进血管生成和减少炎症. 失调的TGFβ-1信号传递可能会通过影响胎儿血管分支而导致胎盘病理,如孕前.

科学领域:

生殖生物学生殖生物学
血管生物学血管生物学
蜂信号传输是如何进行的

背景情况:

胎盘周细胞对于胎儿血管发育和血管生成至关重要.
转化生长因子β-1 (TGFβ-1) 信号传递对胎盘发育至关重要,但它在胎盘细胞周细胞功能中的作用尚未得到充分研究.
在孕前症模型中观察到变化的细胞周覆盖和血管分支.

研究的目的:

为了研究TGFβ-1信号传递对人类胎盘周细胞的体外影响.
评估TGFβ-1对周细胞群动力学,血管新生和炎症分泌,ECM产生和细胞能力的影响.

主要方法:

隔离的人类胎盘周细胞被TGFβ-1治疗.
分析包括血管性/炎症性分泌物 (VEGFA,MMP-2,ANG-1,sVCAM-1,IL-6,MCP-1),ECM产生和细胞分析.
研究了TGFβ-1受体ALK5和ALK1.1的参与.

主要成果:

TGFβ-1治疗诱导了亲血管性表型,增加了VEGFA和MMP-2,并减少了ANG-1分泌.
TGFβ-1减弱了炎症标记物 (sVCAM-1,IL-6,MCP-1) 的分泌,并降低了细胞的能力.
一些TGFβ-1效应依赖ALK5,而另一些则表明ALK1参与.

结论:

TGFβ-1信号调节着胎盘周细胞的功能,影响血管生成和炎症反应.
在胎盘细胞中失调的TGFβ-1信号传递可能导致胎盘病理中胎儿血管分支受损,包括孕前.
这些发现突出了TGFβ-1作为潜在的治疗位疾病的目标.

更多相关视频

Author Spotlight: Advancing Research Through Single Cell Sequencing and Spatial Histology in Placental Tissues

Author Spotlight: Advancing Research Through Single Cell Sequencing and Spatial Histology in Placental Tissues

Published on: September 8, 2023

A High Output Method to Isolate Cerebral Pericytes from Mouse

A High Output Method to Isolate Cerebral Pericytes from Mouse

Published on: January 14, 2020

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Role of Hematopoietic Growth Factors

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...