Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Translesion DNA Polymerases02:10

Translesion DNA Polymerases

11.0K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
11.0K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

11.7K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
11.7K
Translation01:31

Translation

155.2K
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...
155.2K
Translation01:31

Translation

17.5K
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are...
17.5K
Alternative RNA Splicing02:18

Alternative RNA Splicing

24.7K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.7K
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

18.1K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
18.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Spatial ecology of breast cancer reveals co-evolution of proliferative and dormant niches.

Genome medicine·2026
Same author

iPSC Neurodegenerative Disease Initiative isogenic CAG repeat iPSC line for Huntington's disease.

bioRxiv : the preprint server for biology·2026
Same author

An empirical Bayes framework for burden and dispersion association tests helps prioritize rare variants associated with Alzheimer's disease.

medRxiv : the preprint server for health sciences·2026
Same author

A class of deep intronic <i>IGHMBP2</i> variants activate a shared cryptic splice donor, enabling correction of select variants with a single antisense oligonucleotide.

medRxiv : the preprint server for health sciences·2026
Same author

A TAD-informed aging-brain xQTL atlas of multi-modal and cell-type-resolved regulatory variation.

medRxiv : the preprint server for health sciences·2026
Same author

Glucose concentration of neuronal media formulations influences PINK1-dependent mitophagy in human iNeurons.

Autophagy reports·2026

相关实验视频

Updated: Jan 14, 2026

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae
07:14

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

Published on: February 25, 2022

6.5K

在ALS/FTD中,TDP-43的损失会诱导神秘的多化.

Sam Bryce-Smith1, Anna-Leigh Brown1, Max Z Y J Chien1,2

  • 1UCL Queen Square Motor Neuron Disease Centre, Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology, University College London, London, UK.

Nature neuroscience
|October 21, 2025
PubMed
概括

在肌缩侧面硬化症 (ALS) 中,TDP-43的核耗尽会导致神秘的替代多基化 (APA) 事件. 这些事件影响RNA稳定性和翻译,揭示了TDP-43功能障碍的新后果.

更多相关视频

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles
06:58

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles

Published on: October 18, 2024

1.2K
Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques
08:16

Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques

Published on: January 12, 2024

1.4K

相关实验视频

Last Updated: Jan 14, 2026

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae
07:14

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

Published on: February 25, 2022

6.5K
Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles
06:58

Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles

Published on: October 18, 2024

1.2K
Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques
08:16

Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques

Published on: January 12, 2024

1.4K

科学领域:

  • 神经科学是一个神经科学.
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 核TDP-43枯竭和细胞质聚合是ALS的标志.
  • 已知TDP-43的损失会导致神秘的外去压缩.
  • 由于TDP-43损失引起的神秘替代多化 (APA) 事件尚未得到充分研究.

研究的目的:

  • 开发一个生物信息管道来识别神秘的APA事件.
  • 为了研究诱导多能干细胞 (iPSC) 衍生神经元与TDP-43损失的神秘APA.
  • 分析ALS和前性痴呆症 (FTD) 患者死后脑组织中神秘APA的发生情况.

主要方法:

  • 生物信息管道用于识别替代的最后一个外原体,3'UTR扩展和内基多化APA.
  • 在iPSC衍生的神经元上进行RNA测序 (RNA-seq),SLAM-seq和核糖体分析 (Ribo-seq).
  • 对TDP-43结合位点的分析. 在神秘的APA位点进行缩.

主要成果:

  • 在缺少TDP-43.3的iPSC衍生神经元中,确定了神秘的APA事件,包括替代的最后的外显子,3'UTR扩展和内在的多基解,在iPSC衍生神经元中.
  • 在这些神秘的APA网站上,TDP-43的绑定站点得到了丰富,TDP-43在APA中显示了抑制和增强的角色.
  • 在ALS和FTD患者的死后脑组织中也发现了所有类型的神秘APA.
  • 不同的加密APA类别不同影响转录水平,RNA稳定性和翻译,3'UTR扩展增加RNA稳定性和翻译.

结论:

  • TDP-43的核耗尽诱导了一系列神秘的APA事件.
  • 这些神秘的APA事件对RNA调节和蛋白质生产有显著的下游影响.
  • 这些发现扩大了TDP-43功能障碍在ALS和FTD中已知的分子后果.