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Updated: Jan 14, 2026

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

Published on: October 6, 2022

草根细菌和生物原银纳米颗粒的生物整合对抗Pseudomonas syringae pv. 注射器导致梅子细菌瘤

Misbah Kanwal¹, Raees Ahmed¹, Asma Hanif²

¹Department of Plant Pathology, Faculty of Agriculture, University of Poonch Rawalakot, Rawalakot, 12350, Pakistan.

BMC plant biology

|October 22, 2025

概括

这项研究探讨了利用有益的土壤细菌和植物衍生的银纳米颗粒来对抗梅子细菌瘤. 一种Bacillus和银纳米粒子的组合有效地减少了疾病,为合成农药提供了天然的替代品.

科学领域:

农业科学农业科学
微生物学微生物学
纳米技术纳米技术

背景情况:

梅种植在经济上具有重要意义,但受到Pseudomonas syringae引起的细菌癌症的威胁.
现有的控制方法面临局限性,需要新的方法.

研究的目的:

优化树枝细菌和生物银纳米粒子 (AgNPs) 的使用,以控制梅子中的细菌瘤.
评估促进植物生长的根茎细菌,并对抗菌有效性进行AgNP的表征.

主要方法:

杆菌被分离,确定 (16S rRNA测序),并评估植物生长促进特征.
生物银纳米粒子 (AgNPs) 通过使用大麻树提取物进行合成和特征 (XRD,FTIR,SEM).
针对P. syringae的AgNPs的抗菌活性在体外进行了测试,随后进行了结合树枝细菌和AgNPs的温室试验.

主要成果:

七个根杆菌分离物表现出生物膜的产生,包括Pseudomonas fluorescens和Bacillus subtilis菌株.
0.6%的生物源性AgNP度显示出最强的抗菌活性,抑制区为24.32毫米.
一个含有0.6%AgNP的Bacillus菌株联盟在温室测试中产生了最低的疾病发病率 (18.13%).

结论:

兼容的树枝细菌联盟和生物银纳米颗粒显示出作为合成农药的有效替代品的潜力.
这种方法提供了一种可持续的策略,用于管理梅园中的细菌瘤.

关键词:

细菌性癌症是一种细菌性癌症.生物控制是生物控制.纳米颗粒纳米颗粒杆菌是一种细菌.在X射线中,X射线的衍射效果是不同的.

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