在人唾液中使用超敏感表面增强拉曼散射在横向流量测定器件中检测内毒素的图形图像级检测
Adewale Adehinmoye1, Daewoo Han2, Pietro Strobbia1
1Department of Chemistry, University of Cincinnati Cincinnati OH 45221 USA.
RSC advances
|October 22, 2025
概括
本研究提出了一种超敏感的定量侧流量测定 (LFA),使用表面增强拉曼散射 (SERS) 来检测唾液中的P. gingivalis内毒素等口腔健康生物标志物.
科学领域:
- 纳米技术纳米技术
- 生物医学诊断 生物医学诊断
- 分析化学 分析化学
背景情况:
- 侧流检测 (LFA) 是广泛使用的快速诊断工具.
- 传统的LFA在灵敏度和量化方面存在局限性.
- 口腔健康生物标志物需要敏感的检测方法.
研究的目的:
- 开发一种超敏感和定量的LFA用于检测P. gingivalis内毒素.
- 使用表面增强拉曼散射 (SERS) 提高LFA灵敏度.
- 建立一种可靠的方法来检测唾液中的口腔健康生物标志物.
主要方法:
- 开发了一种新型LFA设备,其中包含银金纳米恒星 (Ag@AuNS).
- 结合Ag@AuNS与拉曼记者染料分子用于SERS检测.
- 优化唾液样本预处理和LFA磁带设计,以提高性能.
主要成果:
- 取得了P. gingivalis内毒素的图谱水平检测.
- 使用 SERS (18 pg mL-1) 证明了检测极限 (LOD) 的显著改善.
- 通过控制流速和膜接触,提高LFA的灵敏度和一致性.
结论:
- 开发的基于SERS的LFA提供了对口腔健康生物标志物的超敏感和定量检测.
- 这项技术显著改善了传统的LFA限制.
- 这种新型LFA设备有可能改善口腔疾病的诊断和监测.
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