从储存的红细胞中快速释放的氧气可以通过精制的添加剂溶液更长时间保存
Julija Rabcuka1, Athinoula Meli2, Jennifer Jolley2
1Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
Transfusion
|October 22, 2025
概括
像SOLX这样的新增剂解决方案在储存期间更好地保护红细胞 (RBC). 与标准的SAGM相比,SOLX和PAGGSM改善了氧气释放和ATP水平,提高了输血疗效.
科学领域:
- 血液银行和输血医疗服务
- 红细胞生理学 红细胞生理学
- 生物材料和储存解决方案
背景情况:
- 储存的红细胞 (RBC) 丧失了氧气输送的关键功能,影响了输血的有效性.
- 标准的盐酸-腺素-葡萄糖-曼尼托尔 (SAGM) 储存具有局限性.
- 先进的添加剂解决方案,如酸-腺素-葡萄糖-瓜诺辛-盐酸-曼尼托尔 (PAGGSM) 和SOLX (AS-7) 提供了更好的红细胞保存.
研究的目的:
- 评估不同添加剂溶液在长时间储存期间保持红细胞功能的有效性.
- 为了比较储存在SAGM,PAGGSM和SOLX中的红细胞的氧气卸载动力学和代谢完整性.
- 评估FlowScore作为RBC氧气释放的替代标记物的实用性.
主要方法:
- 红细胞缩物在SAGM,PAGGSM或SOLX中储存了56天.
- 血液溶解,腺5'-三酸盐 (ATP) 含量和氧气卸载动力学被定期测量.
- 使用单细胞氧和成像和血液学分析仪上的FlowScore来评估氧气释放.
主要成果:
- 所有储存溶液的血液溶解保持在0.8%的值以下,直到第49天;SOLX在后期阶段的遵守性更好.
- 与SAGM和PAGGSM相比,SOLX存储的RBC保留了更高的ATP水平.
- 在SOLX和PAGGSM存储的RBC与SAGM中的氧气卸载速度更快,FlowScore与这些发现相关.
- 储存SOLX将氧气释放动力学下降的速度减半,在第3周观察到显著的好处.
结论:
- 在56天的时间里,SOLX和PAGGSM与SAGM相比,显著提高了红细胞氧释放动力学.
- 存储在SOLX中的红细胞显示出ATP水平和血液溶解符合性的优异维护.
- 基于流细胞计的FlowScore作为一个可靠的替代品来评估RBC存储中的添加剂溶液性能.
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